Biochemical and Molecular Evaluation of the Plant Ecballium elaterium Extract Effects on Escherichia coli

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Awni Abu-Hijleh
Ghaleb Adwan
Wafa Abdat

Abstract

Aims: This study was conducted to evaluate the genotoxic effects of fruit and leaf ethanolic extracts of Ecaballium elateruim on clinical and reference strains of E. coli (E. coli ATCC 25922).

Methodology: The genotoxic effects of fruit and leaf ethanolic extracts were determined by using enterobacterial repetitive intergenic consensus (ERIC-PCR) and sodium dodecyl sulfate polyacrylamide gel electrophoresis (SDS-PAGE).

Results: The results showed an alteration in DNA and protein profiles of both tested Escherichia coli strains treated with fruit and leaf extracts compared with untreated control. The alterations ranged between decreased or increased intensity of some bands, absence or appearance of new amplified fragments. Moreover, increased concentrations of E. elaterium extracts and increased time intervals seems to yield a more profound increase in total protein concentrations in both tested E. coli strains.

Conclusions: Such findings strongly indicate the genotoxic effects of E. elaterium extracts on both E. coli strains. The results draw attention to the unsafe, improper use of E. elaterium extracts in folkloric medicine and point out the capability of using E. elaterium to treat E. coli infections. Future studies should be needed to find out the exact mechanisms responsible for the observed genotoxicity.

Keywords:
Ecballium elaterium, genotoxic effect, ERIC-PCR, SDS-PAGE.

Article Details

How to Cite
Abu-Hijleh, A., Adwan, G., & Abdat, W. (2018). Biochemical and Molecular Evaluation of the Plant Ecballium elaterium Extract Effects on Escherichia coli. Journal of Advances in Biology & Biotechnology, 19(2), 1-11. https://doi.org/10.9734/JABB/2018/43882
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Original Research Article