Experimental Design for Optimization of β-Xylosidase Production by A. fumigatus Isolated from the Atlantic Forest (Brazil)

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Fabíola Giovanna Nesello Vieira
Divair Christ
Luciana Graciano
Juliana Moço Corrêa
Marina Kimiko Kadowaki
José Luis da Conceição Silva
Rinaldo Ferreira Gandra
Alexandre Maller
Maria de Lourdes Teixeira de Moraes Poli
Rita de Cássia Garcia Simão


The production of β-Xylosidase by a new strain of Aspergillus fumigatus (PC-7S-2 M), isolated from the Brazilian Atlantic Forest, was analyzed at 28°C using modified Czapeck media supplemented with different agroindustrial residues at 1% (w/v). Conidia were inoculated for 7 days, and the best activity for β- Xylosidase was obtained in the presence of barley brewing residue after 4 days (15 U mL-1). To optimize the production of β-Xylosidase, this carbon source was used for a central composite rotational design (CCRD) to obtain a significance level of p < 0.10, which predicted an enzyme activity of 245.04 U mL-1. The model validation revealed β-Xylosidase activity was optimized at 229.06 U mL-1. Furthermore, the production of intracellular A. fumigatus β-Xylosidase increased by 1,500% (15 times) over that initially obtained, achieving 93.47% of the predicted model. This finding obtained during two years emphasizes the availability of A. fumigatus β-Xylosidase production with possible applications in biotechnological processes.

Barley brewing residue, experimental design, agroindustrial residue, hemicellulose, β-Xylosidase, Aspergillus fumigatus

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Vieira, F., Christ, D., Graciano, L., Corrêa, J., Kadowaki, M., Silva, J., Gandra, R., Maller, A., Moraes Poli, M. de L., & Simão, R. (2019). Experimental Design for Optimization of β-Xylosidase Production by A. fumigatus Isolated from the Atlantic Forest (Brazil). Journal of Advances in Biology & Biotechnology, 21(3), 1-16. https://doi.org/10.9734/jabb/2019/v21i330093
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