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Aim: This study was to investigate the effects of apple cider vinegar with “the mother” on lipid profile and electrolytes of Wistar rats.
Materials and Methods: Twelve female albino rats with mean weight of 150±20 were grouped into four groups. The first group was the control. The control was given distilled water and allowed access to normal animal feed ad libitum but was not administered apple cider vinegar. The second group was the group to be sacrificed after the first week of experiment. The group was given distilled water, allowed access to normal animal feed ad libitum and administered 1ml apple cider vinegar solution twice daily. The third group was the group to be sacrificed after the second week of experiment. The group had same treatment as the second group above. The fourth group was the group to be sacrificed after the third week which was the final week of experiment. The group had same treatment like the second and third groups.
Results: After oral administration of the apple cider vinegar on rats for 7 days up to 21 days, the results revealed that the significant reductions in a time dependent manner with the highest reductions obtained on the last week of experiment (p<0.05). After 21 days, triglycerides reduced from 3.37 ± 0.14 to 2.73 ± 0.13 mmol/l, total cholesterol from 4.04 ± 0.98 to 3.62 ± 0.33, low density lipoprotein cholesterol from 8.24 ± 1.31 to 7.02 ± 0.30, very low density lipoprotein cholesterol from 1.55 ± 0.07 to 1.42 ± 0.04mmol/l in the blood of rats. It also revealed a significant decrease (p< 0.05) in calcium electrolyte concentration from 11.54 ± 0.21 to 7.09 ± 0.20 mmol/l. It also revealed significant decrease (p<0.05) in the sodium and elevation in potassium electrolytes concentrations from 153.63 ±0.24 to 120.30 ± 1.31 and 3.61 ± 0.30 to 4.92± 0.46 mmol/l respectively.
Conclusion: The results suggested that the apple cider vinegar reduced triglycerides and cholesterol levels in the blood of Wistar rats. The results also suggested that apple cider vinegar reduced calcium and sodium electrolyte levels in the blood but increased potassium levels in the blood of Wistar rats based on the 1ml administration for 21 days.
Saladin K. Anatomy and physiology. The Unity of Form and Function. McGraw-Hill Education. 2015;55.
Amin T, HamedI, Read A, Matar A. The effect of apple cider vinegar and grape vinegar on lipid profile in albino white rats. Jordan J of Pharmaceu Sci. 2014;7:3.
Raskin I, Ripoll C. Can Apple a day keep the doctor away? In Current Pharmaceutical Design. 2014;10:3419-3429.
Claiborne C. The New York times cook book, New York. Harper and Brothers. 1961;530.
Johnston CS, Gaas CA. Vinegar medicinal uses and anti glycemic effect. Med. Gen. Med. 2006;8(2):61.
Dornelles M, Guerreiro-Tanomaru JM, de Faria-Junior NB, Hungaro-Duarte MA, Kuga MC, Tanomaru-Filho M. Antibacterial efficacy of endodonntic irrigating solutions and their conbinations in root canals contaminated with Enterococcus faecalis. Oral Sur. Oral Med. Pathol. Oral Radiol. Endod. 2011;112(3):396-400.
Shishehbor F, Mansoori A, Sarkaki AR, Jalali MT, Latifi SM. Apple cider vinegar attenuates lipid profile in normal and diabetic rats. Pakistan Journal of Biological Sciences. 2008;11(23):2634-2638.
Beheshti Z, Yiong HC, Hamid SN, Fatemeh H, Rogheyeh N, Mohammad S, Mohammad TS. Influence of apple cider vinegar on blood lipids. Life Science Journal. 2012;9(4):2431-2440.
Simons LA, Gibson JC. Lipid: Clinicians guide. 1stEdition. MTP Press Limited, England. 1980;81.
Ellefson RD, Caraway WT. Fundamentals of clinical chemistry, 2nd Edition. Heineman Books, London. 1976;457.
Dolphin PJ. Origin of aplioprotein A-1 polymorphism in plasma. Clin. J. Biochem. Cell Bio. 1985;63:850-869.
Nelson RH. Hyperlipidemia as a risk factor for cardiovascular disease. Prime Care: 2013;40(1):55.
Murray RD, Horfield JE, Mccormick WD. Historical and current perspectives on the treatment, control and pathogenesis of milk fever in diary cattle. Vet Rec. 2008; 163(19):561-565.
Harris W, Levey J. The new Columbia encyclopaedia, 4th Edition. New York City: Columbia University. 1975;155.
Tietz NW. Clinical guide to laboratory tests, 3rd edn. WB Saunders Co. Philadelphia, PA. 1995;874.
Trinder P. In vitro determination of sodium in serum. Analyst. 1951;76:596.
Zubay GL. A text on biochemistry. 4th Editon. Wm. C., Brown Publisher, Columbia University. 1998;543-550.
Mahbubeh S, Sedighe A, Akram E, Ali, Haeri R, Nafiseh E. Effects of apple juice on risk factors of lipid profile, inflammation and coagulation, endothelial markers and artherosclerotic lesions in high cholesterolemic rabbits. Health and Disease. 2009;9:10.
Fushismi T, Suruga T, Oshima Y, Fukiharu M, Tsukamoto Y, Goda T. Dietary acetic acid reduces serum cholesterol and triacylglycerol in rats fed a cholesterol-rich Diet. Brj. Nutri. 2006;95:916-24.
Fredrickson DS, Gotto AM, Levy RI. In the metabolic basis of inherited disease. 3rd Edition. McGraw-Hill Book Co. New York. 1972;545.
Budak NH, Duygu KD, Cagri MS, Atif CS, Tugba KT, Metin IC, Zeynep BG. Effects of apple cider vinegars produced with different techniques on blood lipids in high-cholesterol fed rats. J. Agric. Food Chem. 2011;59(12):6638-6644.
Ajaykumar TV, Anandarajagopal K, Jainaf RAM, Venkateshan N, Anath R. Effect of apple cider vinegar on lipid profiles. Inter J of Bio and Pharmaceu Resear. 2012;3(8): 942-945.
Allgeier RJ. Newer development in vinegar manufacture (Manufacture of white or spirit vinegar), Umbreit WW. Advances in Micro-biology. Elsevier Academic Press, Inc. 1960;2.
Naziroglu M, Guler MO, Saydam G, Kucukayaz M, Sozbir E. Apple cider vinegar modulates serum lipid profile, erythrocyte, kidney, and liver membrane oxidative stress in ovariectomized mice fed high-cholesterol. J Membr. Biol. 2014; 247(8):667-73.
Halima BH, Khlifi S, Jemaa BH, Gara SH, Abdallah A. Antihyperglycemic, anti-hyperlipidemic and modulatory effects of apple cider vinegar on digestive enzymes in experimental diabetic rats. Inter J of Pharmacol. 2016;12:505-513.
Bouderbala H, Kaddouri H, Kheroua O, Saidi D. Anti-obesogenic effect of apple cider vinegars in rats. Anales Cardiologieet D’angelogie. 2016;65(3):208-13.
Stryer L. A text on biochemistry. 3rd Edition. Stanford University. W.H., Freeman and Company, New York. 1988; 933-934.