Anti-cytoskeleton Immunoscreening of Trypanosoma brucei Expression Library Reveals Novel Immunogenic Conserved Putative Proteins

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Begumisa Godfrey Magyezi
Okalang Uthman
Musisi Kenneth
Wampadde Eddie Mwijjwiga
Lubega W. George

Abstract

Background: The overall shape of the trypanosome is defined by an internal cytoskeleton consisting of a network of microtubules that are cross linked both to each other and the inner face of the plasma membrane. However, the total compliment and identity of the trypanosome cytoskeleton proteins are not yet fully determined despite the fact that some of them may be good targets for diagnostics, drugs and/or vaccines discovery.

Methods: In this study, rabbit anti-Trypanosoma brucei detergent insoluble cytoskeleton sera were produced in vivo and used to probe a T. brucei expression library. The picked plaques were made clonal by a series of library screening followed by PCR amplification, cDNA sequencing and identification of the proteins coded by these sequences using BLAST.

Results: The previously well-known cytoskeleton proteins (paraflagella rod protein and histone H2B), putative cytoskeleton proteins (Dynein light chain and nucleoporin), conserved hypothetical protein (Tb10.61.2430) and novel cytoskeleton protein coding cDNA sequences (not in the sequenced and published T. brucei genome) were identified in this study.

Conclusion: This approach is therefore, useable in the search for novel proteins whose utility in the design and development of diagnostics, drugs and/or vaccines can further be studied.

Keywords:
Antibodies, cDNA sequencing, cytoskeleton, expression library, immune sera, immunoscreening, PCR, proteins, Trypanosoma brucei

Article Details

How to Cite
Magyezi, B., Uthman, O., Kenneth, M., Mwijjwiga, W., & George, L. (2019). Anti-cytoskeleton Immunoscreening of Trypanosoma brucei Expression Library Reveals Novel Immunogenic Conserved Putative Proteins. Journal of Advances in Biology & Biotechnology, 22(1), 1-11. https://doi.org/10.9734/jabb/2019/v22i130104
Section
Original Research Article

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