The In vivo Antioxidant Protective Activity of Mangifera indica Cold Aqueous Leaf Extract in Drosophila Melanogaster

Main Article Content

Etuh, Monday Alexander
John C. Aguiyi
Ochala, Sunshine Ogwu
Omale Simeon
Oyeniran, Oluwatosin Imoleayo
Oshibanjo Olusegun Debola
Dung Pam

Abstract

Objective: To evaluate in vivo antioxidant activity of Mangifera indica cold aqueous leaf extract. 

Methods: A number of 50 adult flies were exposed to graded concentrations of Mangifera indca cold aqueous leaf extract, 2.5 mg/10 g diet, 5 mg/10 g diet and 10 mg/10 g diet for 5 days. Each concentration was prepared in 200 µl of distilled water and replicated five times. 10 g diet with 200 µl distilled water served as control. Mortality reading was taken at 24 hours interval. The flies were homogenized, centrifuged and the supernatant was used to assay for Glutathione-S-transferase (GST), Catalase (CAT) and Total thiol content.

Results: The % mortality of flies after 5 days showed 32.5%, 0%, 15.5% and 37% in the control (10 g diet with 200 µl of distilled water), 2.5 mg/10 g diet, 5 mg/10 g diet and 10 mg/ 10 g diet respectively. There was elevation in total thiol content and high GST and CAT activity in 2.5 mg/10 g diet and 5 mg/10 g diet treated flies.

Conclusion: The 100% and 85% survival of 2.5 mg/10 g and 5 mg/10 g diet-treated flies respectively and increase of fly antioxidant system after 5 days exposure at these concentrations may suggest protective activity of Mangifera indica in D. melanogaster.

Keywords:
In vivo, antioxidant activity, cold aqueous extract, drosophila melanogaste, Mangifera indica, catalase, total thiol, glutathione-S-transferase.

Article Details

How to Cite
Alexander, E., C. Aguiyi, J., Ogwu, O., Simeon, O., Imoleayo, O., Debola, O., & Pam, D. (2019). The In vivo Antioxidant Protective Activity of Mangifera indica Cold Aqueous Leaf Extract in Drosophila Melanogaster. Journal of Advances in Biology & Biotechnology, 22(2), 1-7. https://doi.org/10.9734/jabb/2019/v22i230108
Section
Short communication

References

Jayachitra A, Krithiga N.“Study on anti-oxidant property in selected medicinal plant extract. International Journal of Medicinal and Aromatic Plants. 2010;2(3): 495-500.

Khalaf NA, Shakya AK, Al-Othman A, El-Agbar Z, Farah H. Antioxidant activity of some common plants. Turkish Journal of Biology. 2008;32(1):51-55.

Patel VR, Patel PR, Kajal SS.“Antioxidant activity of some selected medicinal plants in western region India.”Advances in Biological Research. 2010;4:23-26.

Shahidi F, Wanasundara PK. “Phenolic antioxidants. Critical Reviews in Food Science and Nutrition. 1992;32(1):67-103.

Büyükokuroǧlu ME, Gülçin I, Oktay M, Küfrevioǧlu ÖI. In vitro antioxidant properties of dantrolene sodium. Phar-macological Research. 2001;44:6:491-494.

Sies H. Oxidative stress: From basic research to clinical application. Am J Med. 1911;91:31-9.

Jones DP, Carlson JL, Mody VC, et al. Redox state of glutathione in human plasma. Free Radic Biol Med. 2000;28: 625-35.

Halliwell B, Gutteridge JM. The anti-oxidants of human extracellular fluids. Arch Biochem Biophys. 1990;280:1-8.

Tharanathan RN, Yashoda HM, Prabha TN. Mango (Mangifera indica L.), the king of fruits A review. Food Reviews International. 2006;22:95-123.

Jahurul MHA, Zaidul ISM, Ghafoor K, Al-Juhaimi FA, Nyam KL, Norulaini NAN, Sahena F, Omar AKM. Mango (Mangifera indica L.) by-products and their valuable components: A review. Food Chemistry. 2015;183:173-180.

Ethno medicinal use of mango; 2016.
Available:http://naturalhomeremedies.co/Mango.html.

Nunez-Selles AJ. Antioxidant therapy: Myth or reality? J. Braz. Chem. Soc. 2005;16(4):101 -108.

Kohler RE. Lords of the fly: Drosophila genetics and the experimental life. University of Chicago Press; Chicago, IL, USA. 1994;xv:321.

Ellman GL. Tissue sulfhydryl groups, Arch. Biochem. Biophys. 1959;82(1):70-77. Available:http://dx.doi.org/10.1016/0003-9861 (59)90090-6 13650640

Habig WH, Jakoby WB. Assays for differentiation of glutathione S-transferases, Methods Enzymol. 1981;77:398-405.

Aebi H. Catalase in vitro, methods enzymol. 1984;105:121-126.

Jones DP, Carlson JL, Mody VC, et al. Redox state of glutathione in human plasma. Free Radical Biological Medical. 2000;28:625-35.

Schafer FQGR. Buettner redox environ-ment of the cell as viewed through the redox state of the glutathione disulfide/ glutathione couple. Free Radica Biological Medical. 2001;30:1191-212.

Chelikani P, Fita I, Loewen PC. Diversity of structures and properties among catalases. Cell Molecular Life Science. 2004;61(2): 192-208

Karuppanan M, Krishnan M, Padarthi P, Namasivayam E. Hepatoprotective and antioxidant effect of Mangifera indica leaf extracts against mercuric chloride-induced liver toxicity in mice. Euroasian Journal of Hepato-gastroenterology. 2014;4(1):18.

Zhang ZS, Wen SP, Wang H, Shao C, Hu S. Study on antisenescence potential of Rosmarinus officinalia extract. Journal of Food Research and Development. 2012;3.

Lu Y, Yeap FL. Antioxidant activities of polyphenols from sage (Salvia officinalis). Food Chem. 2001;75:197–202.

Zhao GR, Xiang ZJ, Ye TX, Yuan YJ, Guo ZX. Antioxidant activities of salvia miltiorrhiza and panax notoginseng. Food Chem. 2006;99:767-774.

Javanmardi J, Tushnof SFC, Locke E, Vivanco JM. Antioxidant activity and total phenolic content of Iranian ocimum accessions. Food Chem. 2003;83:547-550.