Exploring the Antioxidant Potential of Fagopyrum esculentum Moench (Common Buckwheat) Leaf Extract
Priya Chand
Department of Molecular Biology and Genetic Engineering, C.B.S.H., G.B.P.U.A.&T, Pantnagar, 263145, Uttarakhand, India.
Sonu Ambwani *
Department of Molecular Biology and Genetic Engineering, C.B.S.H., G.B.P.U.A.&T, Pantnagar, 263145, Uttarakhand, India.
Brij Raj Singh
Department of Molecular Biology and Genetic Engineering, C.B.S.H., G.B.P.U.A.&T, Pantnagar, 263145, Uttarakhand, India.
Tanuj Kumar Ambwani
Department of Veterinary Physiology and Biochemistry, C.V.A.S., G.B.P.U.A.&T., Pantnagar, 263145, Uttarakhand, India.
Ashutosh Dubey
Department of Biochemistry, C.B.S.H., G.B.P.U.A.&T, Pantnagar, 263145, Uttarakhand, India.
*Author to whom correspondence should be addressed.
Abstract
This study was conducted in 2022 at the Department of Molecular Biology and Genetic Engineering, College of Basic Sciences and Humanities, G.B. Pant University of Agriculture & Technology, Pantnagar, Uttarakhand, India. The objective was to assess the antioxidant potential of the hydromethanolic leaf extract of Fagopyrum esculentum Moench (common buckwheat) by evaluating its reducing power and metal ion chelation abilities. Leaves of Fagopyrum esculentum were collected from the Pithoragarh district in Uttarakhand and authenticated. A 50% hydromethanolic extract (FEE) was prepared from the powdered leaves. The antioxidant potential of FEE was assessed using reducing power and metal ion chelation tests. The hydromethanolic extract yielded 7.17%. FEE exhibited significant antioxidant activity in a concentration-dependent manner, demonstrating both electron-donating ability and metal ion chelation capacity with an IC50 value of 108.846 µg/ml. These results highlight the potential of F. esculentum leaf extract as a natural antioxidant for use in the pharmaceutical, cosmetic, and food industries. Further studies are recommended to investigate its effects In vivo.
Keywords: Fagopyrum esculentum, buckwheat, antioxidant activity, reducing power, metal ion chelation, leaf extract