First Report of Banana Streak Virus Infecting Banana in Burkina Faso
Bakary Ouattara
*
Centre National de Recherche Scientifique et Technologique, Institut de l’Environnement et de Recherches Agricoles, Laboratoire de Protection et Défense des Cultures, 01 BP 476 Ouagadougou, Burkina Faso.
Moustapha Koala
Centre National de Recherche Scientifique et Technologique, Institut de l’Environnement et de Recherches Agricoles, Laboratoire de Protection et Défense des Cultures, 01 BP 476 Ouagadougou, Burkina Faso and Agence Nationale de Biosécurité, Laboratoire National de Biosécurité, 06 BP 10798 Ouagadougou, Burkina Faso.
Emmanuel Kaboré
Centre National de Recherche Scientifique et Technologique, Institut de l’Environnement et de Recherches Agricoles, Laboratoire de Protection et Défense des Cultures, 01 BP 476 Ouagadougou, Burkina Faso.
Hermann Tapsoba
Centre National de Recherche Scientifique et Technologique, Institut de l’Environnement et de Recherches Agricoles, Laboratoire de Protection et Défense des Cultures, 01 BP 476 Ouagadougou, Burkina Faso.
Thyérry Kiébè Dakouo
Centre National de Recherche Scientifique et Technologique, Institut de l’Environnement et de Recherches Agricoles, Laboratoire de Protection et Défense des Cultures, 01 BP 476 Ouagadougou, Burkina Faso.
Kadidia Koita
Université Joseph KI-ZERBO, Département de Biologie et de Physiologie Végétale, Laboratoire de Biosciences, 03 BP 7021 Ouagadougou 03, Burkina Faso.
Issa Wonni
Centre National de Recherche Scientifique et Technologique, Institut de l’Environnement et de Recherches Agricoles, Laboratoire Central d’Horticulture, 01 BP 910 Bobo Dioulasso, Burkina Faso.
Drissa Sérémé
Centre National de Recherche Scientifique et Technologique, Institut de l’Environnement et de Recherches Agricoles, Laboratoire de Protection et Défense des Cultures, 01 BP 476 Ouagadougou, Burkina Faso.
*Author to whom correspondence should be addressed.
Abstract
Banana plants (Musa spp), both food and economic crops, are potential hosts for a various range of badnavirus species considered a major constraint to banana improvement and a threat to Musa production worldwide. A survey and sample collection were carried out in two main banana-growing regions of Burkina Faso. The samples collected were subjected to biological, serological and molecular diagnosis of the reverse transcriptase/ribonuclease H (RT/RNase H) region using Badna FP/RP primers, followed by sequence comparison with the Genbank database. Analyses confirmed the presence of Banana streak virus (BSV) in all symptomatic samples tested in both regions. Amplification bands of the expected size were obtained for the symptomatic samples tested positive in the serological test. These partial RT/RNase H gene sequences shared highest nucleotide identity ranging from 85.80% to 99.05% with BSV isolates in GenBank. These results are proof of the existence of BSV in Burkina Faso. It is therefore important to undertake studies that will provide basic information on the virus for the development of effective control strategies.
Keywords: Banana, banana streak virus, diagnosis, symptomatic, reverse transcriptase/ribonuclease