Main Article Content
Biocorrosion is a form of corrosion of metallic and concrete materials mediated by microorganisms. Acid producing bacteria are major culprits in the corrosion of materials in the environment. This study focused on the inhibition of biocorrosion by acid producing bacteria using aqueous extract of Phyllanthus amarus (PAAE). Acid producing bacteria were isolated from produced water samples collected from oilfields located in Niger Delta, Nigeria. Multiple fermentation tube technique was adopted for the isolation of the acid producing bacteria using phenol red dextrose broth as culture broth medium. The gravimetric analysis was performed with different concentrations of the plant extract incorporated in universal culture bottles containing broth, sample and carbon steel coupon. The setup was incubated at 20ºC, 30ºC and 40ºC for 7 days and for 14 days. The least corrosion rate (CR) at 20ºC, 30ºC and 40ºC for the 7 days test were 1.446mp/y (80mg/ml PAAE); 27.558mp/y (5 mg/mlLeu+20 mg/ml PAAE) and 5.134 mp/y (80 mg/ml PAAE) with corresponding inhibition efficiency (IE) of 81.92, 87.750 and 80.91 respectively. For the 14 days, the CR values at 20ºC, 30ºC and 40ºC were: 3.192mp/y (5mg/mlLeu+20mg/ml PAAE); 1.458 mp/y (5 mg/mlLeu + 20 mg/ml PAAE) and 117.345mp/y (5 mg/mlLeu + 20 mg/ml PAAE) with corresponding IE of 86.09, 83.87 and 98.89 respectively. The results obtained show that the extract could be considered as a good inhibitor for the biocorrosion of carbon steel mediated by acid producing bacteria.
Little B, Lee J, Ray R. A review of ‘green’ strategies to prevent or mitigate microbiologically influenced corrosion. Biofouling. 2007;23(2):87-97.
Umar M, Mohammed IB, Oko JO, Tafinta IY, Aliko AA, Jobbi DY. Phytochemical analysis and antimicrobial effect of lemon grass (Cymbopogon citratus) obtained from Zaria, Kaduna State, Nigeria. Journal of Complementary and Alternative Medical Research. 2016;1(2):1-8.
Jantan I, Ilangkovan M, Yuandani Mohamad HF. Correlation between the major components of Phyllanthus amarus and Phyllanthus urinaria and their inhibitory effects on phagocytic activity of human neutrophils. BMC Complement Altern Med. 2014;14:429.
Patel JR, Tripathi P, Sharma V, Chauhan NS, Dixit VK. Phyllanthus amarus: ethnomedical uses, phytochemistry and pharmacology: A review. J Ethnopharmacol. 2011;138(2):286–313.
Briggs WF, Agwa OK, Abu GO. Quantitative Microbial Risk Assessment (QMRA) of Groundwater in Abonnema Community in Kalabari Kingdom, Rivers State, Nigeria. Adv Biotech & Micro. 2018; 8(3):55574D.
Ber R, Mamroud E, Aftalion M, Tidhar A, Gur D, Flashner Y, Cohen S. Development of an improved selective agar medium for isolation of Yersinia pestis. Applied and Environmental Microbiology. 2003;69(10): 5787–5792.
Ghosh T, Maity TK, Bose A, Dash GK, Das M. A study on antimicrobial activity of Bacopa monnieri Linn. aerial plants. J. Natural Remedies. 2006;6(2):170-173.
Suryawanshi MA, Mane VB, Kumbhar GB. Methodology to extract essential oils from Lemon grass leaves: Solvent extraction Approach. International Research Journal of Engineering and Technology (IRJET). 2006;03(8):1775-17779.
Dhandapani R, Lakshmi D, Balakrishnan V, Jayakumar S, kumar A. Preliminary phytochemical investigation and antibacterial activity of Phyllanthus amarus Schum & Thorn. Ancient Science of Life. 2007;27(1):1-5.
Ugochukwu SC, Uche A, Ifeanyi O. Preliminary phytochemical screening of different solvent extracts of stem bark and roots of Dennetia tripetala G. Baker. Asian Journal of Plant Science and Research. 2013;3(3):10-13.
Pandey A, Tripathi S. Concept of standardization, extraction and pre phytochemical screening strategies for herbal drug. Journal of Pharmacognosy and Phytochemistry. 2014;2(5):115-119.
Adina ES, Gozescu I, Dabici A, Sfirloaga P, Szabadai Z. Organic Compounds FT-IR Spectroscopy, Macro to Nano Spectroscopy, Dr. Jamal Uddin (Ed.); 2012.
[ISBN: 978 – 953-51-0664-7]
Immanuel OM, Abu GO, Stanley HO. Inhibition of biogenic sulphide production and biocorrosion of carbon steel by sulphate reducing bacteria using Ocimum gratissimum essential oil. Journal of Biology and Biotechnology. 2016;10(2):1-12.
Korenblum E, Goulart FRV, Rodriguues IA, Abrue F, Lins U, et al. Antimicrobial action and anti-corrosion effect against sulphate reducing bacteria by Lemon grass (Cymbopogon citratus) essential oil and its major component ‘the citral’. AMB Express. 2013; 3:44.
Wang SJ, Zhong JJ. Bioreactor engineering. In: Bioprocessing for value-added products renewable resources. Shang-Tian Yang (Ed,). Elsevier Science; 2007.
Altemimi A, Lakhassassi N, Baharlouei, Watson DG, Lightfoot DA. Phytochemicals: Extraction, isolation and identification of bioactive compounds from plant extracts. Plants. 2017;6(4). Pii: E42.
Tiwari P, Kumar B, Kaur M, Kaur G, Kaur H. Phytochemical screening and Extraction: A Review. Internationale Pharmaceutica Sciencia. 2011;1:1.