Chemical Constituents of the Volatile Derived from the Black Garlic Processing

Main Article Content

Hamasuke Hamano
Chihiro Sutoh
Yoshiyuki Osanai
Jin-ichi Sasaki


Pungent gas released from the black garlic production was analyzed by Gas Chromatography to clarify compounds included. Three major constituents as ammonia, sulfide-group and aldehyde-group were identified as stimulating agents. Vegetables (plants) released-gas from garlic, onion, horse radish, et al. showed bacteria killing activity against P. aeruginosa, B. natto, enterohemorrhagic E. coli O157, MRSA and C. albicans. Scanning electron micrographs showed that anti-bacteria mechanisms by the volatile seem different depending on species of vegetables (plants).

Black garlic volatile, chemical constituents of volatile, garlic processing, antibacterial activity.

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How to Cite
Hamano, H., Sutoh, C., Osanai, Y., & Sasaki, J.- ichi. (2019). Chemical Constituents of the Volatile Derived from the Black Garlic Processing. Journal of Advances in Biology & Biotechnology, 22(4), 1-5.
Original Research Article


Sasaki J, Lu C, Machiya E, Tanahashi M, Hamada H. Processed black garlic (Allium sativum) extracts enhance anti-tumor potency against mouse tumors. Medicinal and Aromatic Plant Science and Biotechnology. 2007;1(2):278-281.

Kaneko T, Chihara H, Beppu H, Tonatsu A, Sonoda S, Shimpo K. Effect of the Black Garlic on 1, 2-Dimethylhydrazin-induced Premallignant Lesion in the Rat Colon. (In Japanese) Fujita Gakuen Medical J. 2007; 31(2):143-147.

Sasaki J, Kita T, Ishita K, Uchisawa H, Matsue H. Antibacterial activity of garlic powder against Escherichia coli 0157. J. Nutr Sci Vitaminol. 1999;45:785-790.

Sasaki J. Bioactive Phytocompound and Products Traditionally Used in Japan. in Modern Phytomedicine. Ed. By Iqubal Ahmad, et al. WILEY-VCH (Germany). 2006;79-96.

Sasaki J, Yamanouchi K, Nagaki M, Arima H, Aramachi N, Inaba T. Antibacterial Effect of Lavender Flavor (volatile). J. Food Science and Engineering. 2015;5(2):95-102.

Sasaki J, Yamauchi K. Odor (volatiles) in the plants was a weapon to Fight bacteria.In Antimicrobial research: Novel bioknowledege and educational Programs. ed. By A. Mendez-Vilas (FORMATEX, Spain). 2017;149-154.