Antioxidant Activity of nHexane Extract of Caryota no Seed Using Drosophila melanogaster Model

Main Article Content

Chinonye A. Maduagwuna
Simeon Omale
Monday A. Etuh
Steven S. Gyang

Abstract

Aims: To investigate the anti-oxidant activity of the nhexane extracts of Caryota no seeds in D. melanogaster.

Study Design: Experimental design.

Place and Duration: Sample: African Centre of Excellence for Phytomedicine Research and Development, University of Jos, Jos Plateau State Nigeria between June 2018 and February 2019.

Methodology: Total protein assays were carried out by exposing 50 flies in each vial to the following concentrations: 300 mg, 350 mg, 400 mg, 500 mg and 600 mg of nhexane extracts in 5 replicates for 7 days with daily recording of mortality. In vivo antioxidant activity study was conducted by measuring levels of Glutathione-S-Transferase (GST), catalase (CAT) and total thiol (TT) from supernatants of whole fly homogenates using a spectrophotometer at specific wavelenghts. The values were derived as part of the total protein value. The statistical difference among test groups was presumed at P <0.05.                                   

Results: The nhexane extract of CN caused nonsignificant (P = .52) changes in total protein levels compared to the control. The antioxidant activity showed nonsignificant (P = .64) higher total thiol contents and GST (P = .76) activity in nhexane extract-treated flies and lower catalase (P = .30) activity was recorded in the nhexane extract-treated flies compared to the controls.

Conclusion: It can therefore be concluded that the nhexane extract of Caryota no possess appreciable concentrations of different types of antioxidants.

Keywords:
In vivo, Caryota no, endogenous, exogenous, Drosophila melanogaster.

Article Details

How to Cite
Maduagwuna, C. A., Omale, S., Etuh, M. A., & Gyang, S. S. (2020). Antioxidant Activity of nHexane Extract of Caryota no Seed Using Drosophila melanogaster Model. Journal of Advances in Biology & Biotechnology, 23(4), 39-47. https://doi.org/10.9734/jabb/2020/v23i430151
Section
Original Research Article

References

Jayachitra A, Krithiga N. Study on antioxidant property in selected medicinal plant extract. International Journal of Medicinal and Aromatic Plants. 2010;2(3): 495–500.

Khalaf NA, Shakya AK, Al-Othman A, El-Agbar Z, Farah H. Antioxidant activity of some common plants. Turkish Journal of Biology. 2008;32(1):51–55.

Patel VR, Patel PR, Kajal SS. Antioxidant activity of some selected medicinal plants in western region India. Advances in Biological Research. 2010;4:23–26.

Shahidi F, Wanasundara PK. Phenolic antioxidants. Critical reviews in food science and nutrition. 1992;32(1):67–103.

Valko M, Leibfritz D, Moncol J, Cronin MTD, Mazur M, Telser J. Free radicals and antioxidants in normal physiological functions and human disease. International Journal of Biochemistry and Cell Biology. 2007;39(1):44–84.

Aqil F, Ahmad I, Mehmood Z. Antioxidant and free radical scavenging properties of twelve traditionally used Indian medicinal plants. Turkish Journal of Biology. 2006; 30(3):177–183.

Koopman J, Simpson D, Goetghebeur P, Wilson K, Egorova T, Bruhl J, Govaerts R. World Checklist of Cyperacae. Facilitated by the Royal Botanical Gardens, Kew. 2005;1-223

Accessed 14 October 2014

Available: http://apps.kew.org/wcsp/

Vanaja D, Kavitha S. A Study on the Bio-efficacy of Caryotaurens L. World Journal of Pharmaceutical Research. 2017;6(4): 1381-1398.

Alarco AM, Marcil A, Chen J, Suter B, Thomas D, Whiteway M. Immune-deficient Drosophila melanogaster: A model for the innate immune response to human fungal pathogens. The Journal of Immunology. 2004;172(9):5622-5628.

Lionakis MS, Kontoyiannis DP. The growing promise of Toll-deficient Drosophila melanogaster as a model for studying Aspergillus pathogenesis and treatment. Virulence. 2010;1(6):488-499.

Tolwinski NS. Introduction: Drosophila – A model system for developmental biology. J Dev Biol. 2017;5(3):9

Virot M, Tomao V, Ginies C, Chemat F. Total lipid extraction of food using d-limonene as an alternative to nhexane. Chromatographia. 2008;68(3-4):311-313.

Etuh MA, Aguiyi JC, Ochala SO, Simeon O, Oyeniran OI, Debola OO, Pam D. The In vivo Antioxidant Protective Activity of Mangiferaindica Cold Aqueous Leaf Extract in Drosophila Melanogaster. Journal of Advances in Biology & Biotechnology. 2019;22(2):1-7.

Habig WH, Jakoby WB. Assays for Differentiation of Glutathione S-Transferases. Methods in Enzymology. 1981;77:398-405.

Abolaji AO, Kamdem JP, Lugokenski TH, Farombi EO, Souza, DO, da Silva Loreto EL, Rocha JBT.Ovotoxicants 4-Vinylcyclohexene 1,2-Monoepoxide and 4-Vinylcyclohexene Diepoxide Disrupt Redox Status and Modify Different Electrophile Sensitive Target Enzymes and Genes in Drosophila Melanogaster. Redox Biology. 2015;5:328–39, DOI:10.1016/j.redox.2015.06.001

Ellman GL. Tissue sulfhydryl groups, Arch Biochem Biophys. 1959;82(1):70-77.

Available:http://dx.doi.org/10.1016/0003-9861 (59)90090-6 13650640

Aebi H, Catalase in vitro. Methods Enzymol. 1984;105:121–126 6727660.

Phaniendra A, Jestadi DB, Periyasamy L. Free Radicals: Properties, Sources, Targets, and Their Implication in Various Diseases. Indian Journal of Clinical Biochemistry. 2015;30(1):11-26.

Pham-Huy LA, He H, Pham-Huy C. Free Radicals, Antioxidants in Disease and Health. Int J Biomed Sci. 2008;4(2):89–96.

Onoja SO, Omeh YN, Ezeja MI, Chukwu MN. Evaluation of the In Vitro and In Vivo Antioxidant Potentials of Aframomum Melegueta Methanolic Seed Extract. J Tropical Med. 2014;6:1-6.

ID 159343

DOI:http://dx.doi.org/10.1155/2014/159343

DeLeve L, Kaplowitz N. Glutathione metabolism and its role in hepatotoxicity. PharmacoTher. 1991;52:287–305.

Hwang C, Sinsky AJ, Lodish HF. Oxidized redox state of glutathione in the endoplasmic reticulum. Science 1992;257: 1496–1502.

Prakash M, Shetty MS, Tilak P, Anwar N. Total Thiols: Biomedical importance and their alteration in various disorders. Online Journal of Health and Allied Sciences. 2009;8(2):1–9.

Ferna´ndez-Checa J, Lu SC, Ookhtens M, DeLeve L, Runnegar M et al. The Regulation of Hepatic Glutathione. In: Hepatic Anion Transport and Bile Secretion: Physiology and Pathophysiology, Tavoloni N, Berk PD, eds, Marcel Dekker, New York. 1992;363–95.

Cotgreave IA, Gerdes RC. Recent trends in glutathione biochemistry-glutathione-protein interactions: A molecular link between oxidative stress and cell proliferation? BiochemBiophys Res Commun. 1998;242:1–9.

Mallikarjunappa S, Prakash M. Urine protein thiols in chronic renal failure patients. Indian J Nephrol. 2007;17:7-9.

Markan S, Kohli HS, Sud K et al. Oxidative stress in primary glomerular diseases: A comparative study. Mol Cell Biochem. 2008;311:105-10.

D'Autreaux B, Toledano MB. ROS as signalling molecules: Mechanisms that generate specificity in ROS homeostasis. Nat Rev Mol Cell Biol. 2007;8(10):813-24.

Mates JM, Sanchez-Jimenez F. Antioxidant enzymes and their implications in pathophysiologic processes. Front Biosci. 1999;4:D339-45.

Anas AA, Wiersinga WJ, de Vos AF, van der Poll T. Recent insights into the pathogenesis of bacterial sepsis. Neth J Med. 2010;68(4):147-52.

Webster NR, Nunn JF. Molecular structure of free radicals and their importance in biological reactions. Br J Anaesth. 1988; 60(1):98-108.

Pacher P, Beckman JS, Liaudet L. Nitric oxide and peroxynitrite in health and disease. Physiol Rev. 2007;87(1):315-424.

Abolaji AO, Olaiya CO, Oluwadahunsi OJ, Farombi EO. Dietary consumption of monosodium L-glutamate induces adaptive response and reduction in the life span of Drosophila melanogaster. Cell Biochemistry and Function. 2017;35(3): 164-170.

Abolaji AO, Kamdem JP, Lugokenski TH, Nascimento TK, Waczuk EP, Farombi EO, Loreto EL, Rocha JB. Involvement of oxidative stress in 4-vinylcyclohexene-induced toxicity in Drosophila melanogaster. Free RadicBiol Med. 2014; 71:99–108.