Escherichia coli in Fruit Juice Dispensers

Main Article Content

Ana Claudia Chesca
Ariana da Silva Costa
Carolina Bragine Mangucci

Abstract

Aims: The aim of this study was to evaluate the microbiological quality of juices kept in juice dispensers and sold in retail in canteens and snack bars.

Study Design: The study was done with thirty samples of juice, collected in the morning and in the afternoon, obtained at commercial establishments near Uberaba University, whereas two samples were of cashew juice, two samples of tamarind juice, two samples of passion fruit with mango juice, four samples of guava with acerola juice, eight samples of grape juice and twelve samples of passion fruit juice.

Place and Duration of Study: Food Microbiology Laboratory - University of Uberaba.

Methodology: The temperature at the time of collection was measured. A microbiological analysis was performed. The samples that were positive for thermotolerant coliforms, the analysis was continued, reaching the isolation of Escherichia coli and its subsequent serological identification.

Results: The results point to a contamination, over current legal standards, of 66.7% for the samples collected in the morning and of 73.34% for the samples collected in the afternoon. Conclusion: Greater care in good practices is suggested, especially regarding the personal hygiene of handlers, water quality, sanitation and correct temperature control of fruit juice dispensers.

Keywords:
Classical enteropathogenic E. coli (EPEC), enteroinvader E. coli (EIEC), juice.

Article Details

How to Cite
Chesca, A. C., Costa, A. da S., & Mangucci, C. B. (2020). Escherichia coli in Fruit Juice Dispensers. Journal of Advances in Biology & Biotechnology, 23(11), 1-8. https://doi.org/10.9734/jabb/2020/v23i1130185
Section
Original Research Article

References

Pinheiro AM, Fernandes AG, Fai AEC, Prado GM, Sousa PHM, Maia GA. Chemical, physical-chemical and microbiological evaluation of whole fruit juice: Pineapple, Caju and Passion Fruit. Food Science and Technology, Campinas. 2006;26(1):98-103.

De Marchi R. Development of a passion fruit-based drink (Passiflora edulis Sims. F. Flavicarpa Deg.) With hydrolytic replacement properties. Dissertation (Master) - Faculty of Pharmaceutical Sciences. Paulista State University Julio de Mesquita Filho, Araraquara. 2001;92.

Brazil. Ministry of Health. National Health Surveillance Agency. Ordinance No. 27, of January 13, 1998a. Technical regulation regarding supplementary nutritional information. Official Gazette [of] the Federative Republic of Brazil, DF, Jan 16. 1998.
Available:http://e-legis.anvisa.gov.br/leisref/public/showAct.php?id=97
(Accessed on: 20 Jun 2012)

Brazil. Ministry of Agriculture, Livestock and Supply. Ordinance No. 544, of November 16, 1998b. Approves the Technical Regulations for Setting Identity and Quality Standards, for soft drinks, soft drinks, prepared or liquid concentrates for soft drinks or soft drinks, prepared solid for soft drinks, syrup and tea ready for consumption. Official Gazette [of] the Federative Republic of Brazil, DF, November 17; 1998.
Available:http://extranet.agricultura.gov.br/sislegisconsulta/consultarLegislacao.do?operacao=visualizar&id=1012
(Accessed on: 20 Jun 2020)

Brazil. Ministry of Agriculture, Livestock and Supply. Decree no. 3,510, of June 16, 2000. Amends provisions of the Regulation approved by Decree no. 2,314, of September 4, 1997, which provides for the standardization, classification, registration, inspection, production and inspection of drinks. Official Gazette [of] the Federative Republic of Brazil, DF, June 19. 2000. Available:http://extranet.agricultura.gov.br/sislegisconsulta/consultarLegislacao.do?operacao=visualizar&id=1012
(Accessed on: 20 Jun 2020)

Brazil. Ministry of Health. National Health Surveillance Agency. Resolution - RDC n.259, of September 20, 2002. Technical regulation for the labeling of packaged foods. Official Gazette [of] the Federative Republic of Brazil, DF, 23 September. 2002.
Available:http://e-legis.anvisa.gov.br/leisref/public/showAct.php?id=1261
(Accessed on: 20 Jun 2020)

Brazil. Ministry of Health. National Health Surveillance Agency. Resolution - RDC n.360, of December 23, 2003a. Technical regulation on nutritional labeling of packaged foods. Official Gazette [of] the Federative Republic of Brazil, DF, December 26; 2003.
Available:http://e-legis.anvisa.gov.br/leisref/public/showAct.php?id=9059
(Accessed on: 20 Jun 2020)

Brazil. Ministry of Health. National Health Surveillance Agency. Law No. 10,674, of May 16, 2003b. It requires that commercialized food products that inform about the presence of gluten, as a preventive and control measure for celiac disease. Official Gazette [of] the Federative Republic of Brazil, DF, May 19. 2003.
Available:http://e-legis.anvisa.gov.br/leisref/public/showAct.php?id=58 54
(Accessed on: 20 Jun 2020)

Brazil. Ministry of Health. National Health Surveillance Agency. Resolution - RDC n.359, of December 23, 2003c. Technical regulation on nutritional labeling of packaged foods. Official Gazette [of] the Federative Republic of Brazil, DF, December 26; 2003.
Available:http://e-legis.anvisa.gov.br/leisref/public/showAct.php?id=58 54
(Accessed on: 20 Jun 2012)

Ferrarezi AC. Interpretation of the consumer, evaluation of the purchase intention and the physical-chemical characteristics of the nectar and orange juice ready to drink. 2008. 120 f. Dissertation (Master in Food Science) - Faculty of Pharmaceutical Sciences, Universidade Estadual Paulista Júlio de Mesquita Filho, Araraquara; 2008.

Lee HS, Coates GA. Vitamin C in frozen, fresh squeezed, unpasteurized, polyethylene-bottled orange juice: A storage study. Elsevier Food Chemistry. 1999;65(2):165-168.

Pilon L. Establishment of the useful life of vegetables minimally processed under modified atmosphere and refrigeration. Dissertation for obtaining a Master's degree in Food Science and Technology, presented to the Higher School of Agriculture “Luiz de Queiroz”, University of São Paulo, Piracicaba; 2003.

Franco BDGM, Landgraf M. Food microbiology. São Paulo: Atheneu. 2007; 27-31.

Vanderzant C, Splittstoesser DF. Compendium of methods for the microbiological examination of foods. 3. ed. Washington: American Public Health Association. 1999;1219.

Silva N, et al. Manual of methods of microbiological analysis of food. 3. ed. São Paulo: Varela. 2007;552.

Brazil. Ministry of Health. National Health Surveillance Agency. Ordinance No. 518 of 25 March 2004. Establishes the procedures and responsibilities related to the control and surveillance of the quality of water for human consumption and its standard of drinking water, and other measures. Official Gazette [of] the Federative Republic of Brazil, DF, 26 March; 2004.
Available:http://e-legis.anvisa.gov.br/leisref/public/showAct.php?id=58 54
(Accessed on: 20 Jun 2020)

Brazil. Ministry of Health. National Health Surveillance Agency. Resolution - RDC n.12, of January 2, 2001. Technical Regulation on microbiological standards for food. Official Gazette [of] the Federative Republic of Brazil, Brasília, DF, Jan. 2. 2001.
Available:http://www.anvisa.gov.br/legis/portarias/451-97.htm
(Accessed on: 20 Jun 2020)

Nascimento D, Furlanetto SMP. Quantitative determination of groups of bacteria in fresh orange juices. Revista de Saúde Pública, São Paulo. 1981;15(2).

Oliveira JC, Setti-Perdigão P, Siqueira KAG, Santos AC, Miguel MAL. Microbiological characteristics of fresh orange juice. Food Science and Technology Magazine, Campinas. 2006; 26(2):241-5.

Santos J, Ribeiro GA. Microbiological evaluation of “in natura” orange juices marketed in the city of Pelotas-RS. Food Hygiene Magazine, São Paulo. 2006; 20(138):104-7.

Anders T, et al. Hygienic-sanitary quality of food and beverages produced and / or marketed by food and nutrition units, like canteens, on campuses I and II of the Federal University of Goiás in Goiânia. Magazine of the Brazilian Nutrition Association, São Paulo. 2008;21.

Ruschel CK, Carvalho HH, Souza RB, Tondo EC. Microbiological and physical-chemical quality of orange juices sold on public roads in Porto Alegre-RS. Food Science and Technology, Campinas. 2001; 219(1):94-97.

Sousa MAC, Yuyama LKO, Aguiar JPL, Pantoja L. Açaí juice (Euterpe oleracea Mart.): Microbiological evaluation, heat treatment and shelf life. Health Sciences Journal, Manaus. 2006;36(4): 497-502.

Silva TT, Modesta RCD, Penha EM, Matta VM, Cabral LMC. Organic passion fruit juice processed by microfiltration. Revista de Pesquisa Agropecuária Brasileira, Brasília. 2005;40(4):419-2.

Andrade OMM, et al. Hygienic-sanitary quality of açaí juice sold in 4 neighborhoods of São Luís-MA. Revista Nutrição em Pauta, São Luís. 2007;86:85.

Trabulsi LR, Ordonez JG. Escherichia coli enteropathogenic. In: Trabulsi LR, Alterthum F. Microbiology. São Paulo: Atheneu. 2004;277-288.

Gomes TAT, et al. Prevalence of Escherichia coli strains with localized, diffuse, and aggregative adherence to HeLa cells in infants with diarrhea and matched controls. Journal of Clinical Microbiology, United States. 1989;27:266-269.

Gomes TAT, et al. Enteropathogens associated with acute diarrheal disease in urban infants in São Paulo, Brazil. The Journal of Infectious Diseases, United States. 1991;64:331 – 337.

Nataro JP, Kaper JB. Diarrheogenic Escherichia coli. Clinical Microbiology Reviews, United States. 1998;11:142-201.

Levine MM, Edelman R. Enteropathogenic Escherichia coli of classic serotypes associated with infant diarrhea: Epidemiology and pathogenesis. Epidemiologic Reviews, United States. 1984;6:31-5.

Senerwa D. Enteropathogenic Escherichia coli serotype 0111: HNT isolated from preterm neonates in Nairobi, Kenya. Journal of Clinical Microbiology, United States. 1989;27:1.307-1.311.

Campos LC, et al. Escherichia coli serogroup O111 includes several clones of diarrheogenic strains with diferrent virulence properties. Infection and Immunity, United States. 1994;62(3):282-3.288.

Rodrigues J, et al. Clonal structure and virulence factors in strains of Escherichia coli of the classic serogroup O55. Infection and Immunity, United States. 1996;64: 2.680-2.686.

Scotland SM. Use of gene probes and adhesion tests to characterize Escherichia coli belonging to enteropathogenic serogroups isolated in the United Kingdom. Journal of Medical Microbiology, England. 1996;44:438-443.

Do Valle GR, Gomes A, Irino K, Trabulsi LR. The traditional enteropathogenic Escherichia coli (EPEC) serogroup O125 comprises serotypes which are mainly associated with the category of enteroaggregative E. coli. FEMS Microbiology Letters, England. 1997;152: 95-100.