Nutritional Potential of Spent Brewer's Yeast, A Residual By-Product of Beer Production in Breweries for Future Applications

N’Guessan Yevi Delphine

Laboratory of Biotechnology, Agriculture and Biological Resources Valorisation, Training and Research Unit, Biosciences of University Félix Houphouët-Boigny, Abidjan-Cocody, 22 PO BOX 582, Côte d’Ivoire.

Otchoumou Kraidy Athanase. *

Laboratory of Biotechnology, Agriculture and Biological Resources Valorisation, Training and Research Unit, Biosciences of University Félix Houphouët-Boigny, Abidjan-Cocody, 22 PO BOX 582, Côte d’Ivoire.

Bedikou Ehuie. Micaël

Laboratory of Biotechnology, Agriculture and Biological Resources Valorisation, Training and Research Unit, Biosciences of University Félix Houphouët-Boigny, Abidjan-Cocody, 22 PO BOX 582, Côte d’Ivoire.

Assemian Ines Christelle

Laboratory of Biotechnology, Agriculture and Biological Resources Valorisation, Training and Research Unit, Biosciences of University Félix Houphouët-Boigny, Abidjan-Cocody, 22 PO BOX 582, Côte d’Ivoire.

Ehon Ayawovi Charlotte

Laboratory of Biotechnology, Agriculture and Biological Resources Valorisation, Training and Research Unit, Biosciences of University Félix Houphouët-Boigny, Abidjan-Cocody, 22 PO BOX 582, Côte d’Ivoire.

*Author to whom correspondence should be addressed.


Abstract

The recovery of by-products from food processing chains represents an area of interest in the current world. Waste from beer production, such as beer lees or spent brewer's yeast, constitutes between 1.5 and 3 kg/100 L of beer, representing a source of pollution but a by-product available in quantity. This study aims to characterize the beer lees from breweries and propose possible ways of recovery. The scientific methods have been applied to highlight the nutritional potential of this by-product. Spent brewer's yeast is a high-biological value protein source (46.77%) with a well-balanced amino acid profile. Amino acids (glutamic acid and aspartic acid) and the essential amino acids leucine, lysine and valine are the most abundant, while sulfur amino acids, such as methionine and cysteine, are the least abundant. With a high mineral content (7.3%), the spent brewer's yeast contains significant amounts of manganese 5.54x10-2 mg/k, sodium 3.92x10-2 mg/k, and potassium 3.59x10-2 mg /k. The B complex vitamins are present in large quantities, followed by vitamins C, A and D. Less than 3% of the composition of the spent brewer's yeast is made up of lipids. The most abundant of fatty acids determined for the saturated category is palmitic acid, while oleic acid represents monounsaturated fatty acids, and polyunsaturated fatty acids are represented by linoleic acid and linolenic acid. Carbohydrates (32.77 %) are also important constituents of the dry matter of this by-product. This study showed that spent beer yeast is a source of polyphenol. Spent brewer's yeast has advantages for being incorporated into animal nutrition to meet nutritional needs. Further studies could lead to the production of activated carbon and hydrogen as excellent sources of alternative energy.

Keywords: Brewery waste, spent brewer's yeast, nutritional potential, added value


How to Cite

Delphine, N. Y., Athanase. , O. K., Micaël , B. E., Christelle , A. I., & Charlotte , E. A. (2023). Nutritional Potential of Spent Brewer’s Yeast, A Residual By-Product of Beer Production in Breweries for Future Applications. Journal of Advances in Biology & Biotechnology, 26(9), 30–39. https://doi.org/10.9734/jabb/2023/v26i9654

Downloads

Download data is not yet available.

References

Nayak A, Bhushan B. An overview of the recent trends on the waste valorization techniques for food wastes, J. Environ. Manage. 2019;233:352–370.

Baiano A. Recovery of biomolecules from food wastes – a review, Molecules. 2014; 19:14821–14842.

Caporale G, Monteleone E. Influence of information about manufacturing process on beer acceptability. Food Quality and Preference. 2004;15(3):271-278

Statista Research Department. Production mondiale de bière. 2023;1998-2021

Mussatto SI. Biotechnological potential of brewing industry by-products. In: Singh nee’ Nigam P, Pandey A, editors. Biotechnology for Agro-industrial Residues Utilization. Berlin, Germany: Springer. 2009;313-326

Mathias TRS, Alexandre VMF, Cammarota MC, Mello PPM, Sérvulo EFC. Characterization and determination of brewer’s solid wastes composition. Institute of Brewing & Distilling. 2015;121: 400–404

Briggs DE, Boulton CA, Brookes PA, Stevens R. Brewing: Science and Practice, Woodhead Publishing, Cambridge; 2004.

AOAC. Official methods of analysis. Association of Official Analytical Chemists Ed., Washington DC. 1990;684.

(ISO1871 2009) International Organization for Standardization 1871.. Food and feed products – general guidelines for the determination of nitrogen by Kjeldahl method; 2009 Available:https://www.iso.org/fr/standard/41320.htm

AOAC 938.02. Association of Analytical Communities 938.02. 1938. Glucose in cacao products.Virginia: Association of Official Analytical Chemists, Inc; 1938.

[ICUMSA] International Commission for Uniform Methods of Sugar Analysis. Method GS1-10. The determination of ash in raw sugar by single sulphation; 1998. Available:https://www.icumsa.or g/methods/icumsa-method-gs1-10-1998/

[ICUMSA] International Commission for Uniform Methods of Sugar Analysis. Method GS2/-31.. The determination of iron in refined sugar products and sugar solutions by a colorimetric method; 1994

Available:https://www.icumsa.org/methods/icumsamethod-gs2-3-7-8-31-1994/

[ICUMSA] International Commission for Uniform Methods of Sugar Analysis. Method GS7-15.. The determination of total and soluble phosphate in cane juice by colorimetric method. 1994 Available:https://www.icumsa.org/methods/icumsamethod-gs7-15-1994/

[ICUMSA] International Commission for Uniform Methods of Sugar Analysis. Method GS719. The determination of calcium and magnesium in cane juice and syrup by EDTA titration; 1994 Available from: https://www.icumsa.org/methods/icumsa-methodgs7-19-1994/

[ICUMSA] International Commission for Uniform Methods of Sugar Analysis. Method GS229. The determination of copper refined sugar products by colorimetric method; 1994 Available:https://www.icumsa.org/method-number/gs2-3-291994/

[ICUMSA] International Commission for Uniform Methods of Sugar Analysis. Method GS8/-9.. Determination of calcium in sugar products by EDTA titration; 2000. vailable:https://www.icumsa.org/methods/icumsa-methodgs8-2-3-4-9-2000/

[ICUMSA] International Commission for Uniform Methods of Sugar Analysis. Method GS67. Determination of potassium and sodium in sugar beet by flame photometry; 2007 Available:https://www.icumsa.org/methods/icumsa-method-gs6-7-2007/

Folch J, Lees M, Sloane Stanley GH. A simple method for the isolation and purification of total lipides from animal tissues. Journal of Biology and Chemistry. 1957;226(1):497-509.

Douny C, Tihon, A, Bayonnet, P, Brose F, Degand G, Milet, Jérôme Ribonnet, Laurence Larondelle, Yvan Scippo, M-l . Validation of the Analytical Procedure for the Determination of Malondialdehyde and Three Other Aldehydes in Vegetable Oil Using Liquid Chromatography Coupled to Tandem Mass Spectrometry (LC-MS/MS) and Application to Linseed Oil. In: Food Analytical Methods. Jun 2015;8(6):1425-1435

Soufleros E, Bertrand A. Evaluation d'une méthode CLHP adaptée au dosage des acides aminés du vin. Vitis. 1998 ;37(1): 43-53

European pharmacopoeia book. European pharmacopoeia, 8th Edition, tome. 2014;1: 96-97

Jedlicka A, Klimes J. Determination of Water- and Fat-Soluble Vitamins in Different Matrices Using High-Performance Liquid Chromatography. Chemistry Paper. 2005;59(3):202-222

Singleton VL, Orthofer R, Lamuela-Raventós RM. Analysis of total phenols and other oxidation substrates and antioxidants by means of folin-ciocalteu reagent. Methods in enzymology. 1999; 299:152-178

Tanguler H, Erten H. Utilisation of spent brewer’s yeast for yeast extract production by autolysis: the effect of temperature. Food Bioproducts Processing. 2008;86: 317-321.

Zhu L, Wang J, Feng Y, Yin H, Lai H, Xiao R, He S, Yang Z, He Y. Process Optimization, Amino Acid Composition, and Antioxidant Activities of Protein and Polypeptide Extracted from Waste Beer Yeast. Molecules. 2022;27:6825. Available:https://doi.org/10.3390/ molecules27206825

Vilela ESD, Sgarbieri VC, Alvim ID. Determination of the protein value of intact cells, total autolysate and yeast extract (Saccharomyces sp.). Annual Review of. Nutrition. 2000a;3:185–192.

Yamada EA, Alvim ID, Santucci MCC, Sgarbiere VC. Proximate composition and protein value of residual yeast from ethanolic fermentation and its derivatives. Revista de Nutrição. 2003;16(4):423-432.

Pinto L, Lopes M, Carvalho Filho C, Alves L, Benevides C. Determination of the nutritional value of brewer's yeast (Saccharomyces spp.) derivatives. Brazilian Journal of Agroindustrial Products. 2013;15:7–17.

Franco G. Table of chemical composition of foods, 8th ed., Atheneu Editora, Rio de Janeiro. 1989;92,135,164,196,197.

Amorim M, Pereira JO, Gomes D, Pereira CD, Pinheiro H, Pintado M. Nutritional ingredients from spent brewer’s yeast obtained by hydrolysis and selective membrane filtration integrated in a pilot process. Journal of Food Engineering. 2016;185:42–47.

Berlowska J, Dudkiewicz-Kołodziejska M, Pawlikowska E, Piel- ech-Przybylska K, Balcerek M, Czysowska A, Kregiel D. Utilization of post-fermentation yeasts for yeast extract production by autolysis: the effect of yeast strain and saponin from Quillaja saponaria. Journal of the Institute of Brewing. 2017;123:396–401

Huige NJ. Brewery by-products and effluents. In: Priest FG, Stewart GG (eds) Handbook of Brewing, 2nd edn. CRC Press, Boca Raton; 2006.

Chae HJ, Joo H. In M-J Utilization of brewer’s yeast cells for the production of food-grade yeast extract. Part 1: Effects of different enzymatic treatments on solid and protein recovery and flavor characteristics. Bioresource Technology. 2001;76:253–258

Man-Jin I, Dong CK, Chae HJ. Downstream process for the production of yeast extract using brewer’s yeast cells. Biotechnology and Bioprocess Engineering. 2005;10:85-90.

Caballero-Córdoba GM, Sgarbieri VC. Nutritional and toxicological evalua- tion of yeast (Saccharomyces cerevisiae) biomass and a yeast protein concentrate. Journal of Science Food and Agriculture. 2000;80(3):341–351.

Vieira E, Brandao T, Ferreira IM. Evaluation of Brewer's spent yeast to pro- duce flavor enhancer nucleotides: Influence of serial repitching. Journal of Agricultural and Food Chemistry. 2013; 61(37):8724–8729.

Pancrazio G, Cunha CS, Pinho GP, Loureiro M, Meireles S, Ferreira OVLPMI, Olívia Pinho O. Spent brewer's yeast extract as an ingredient in cooked hams. Meat Science. 2016;121:382–389

Sgarbieri VC, Alvim ID, Vilela ESD, Baldini VLS, Braganolo. Produção piloto de derivados de levedura (Saccharomyces sp.) para uso como ingredients na formulação de alimentos. Brazilian Journal of Food Technology. 1999;2:119-125.

Rakin M, Vukasinovic M, Siler-Marinkovic S. Contribution of lactic acid fermentation to improved nutritive quality vegetable juices enriched with brewer’s yeast autolysate. Food Chemistry 2007;100:599–602.