Journal of Advances in Biology & Biotechnology https://journaljabb.com/index.php/JABB <p style="text-align: justify;"><strong>Journal of Advances in Biology &amp; Biotechnology (ISSN:&nbsp;2394-1081)</strong> aims to publish high quality papers (<a href="/index.php/JABB/general-guideline-for-authors">Click here for Types of paper</a>) in all areas of ‘Biology &amp; Biotechnology’. By not excluding papers on the basis of novelty, this journal facilitates the research and wishes to publish papers as long as they are technically correct and scientifically motivated. The journal also encourages the submission of useful reports of negative results. This is a quality controlled,&nbsp;OPEN&nbsp;peer reviewed, open access INTERNATIONAL journal.</p> SCIENCEDOMAIN international en-US Journal of Advances in Biology & Biotechnology 2394-1081 Phytoremediation, Vermiremediation and Efficiency Assessments of Total Petroleum Hydrocarbons in Contaminated Surface Water from Okpoka Creek, Niger-Delta Nigeria using Cyperus odoratus, Colocasia esculenta, Phoenix roebelenii and Eisenia fetida https://journaljabb.com/index.php/JABB/article/view/30169 <p>The liquid-liquid extraction of Petroleum contaminated surface water samples were conducted in accordance with standard procedure of US EPA. Identification and quantification of TPHs was performed by Gas chromatograph with Mass spectrometric detection (GC/MSD) in accordance with standard analytical method of US EPA 8270;625. The TPHs raw sample showed an elevated value of 104762.42 mg/L above the DPR/EGASPIN maximum contamination limit (MCL). At week 5, TPHs phytoremediations in the monoculture reactors removal efficiency was (31.28 mg/L) 99.97% and mixedculture indicated (19.72 mg/L) 99.98%; their concentrations were above DPR/EGASPIN MCL while polyculture indicated (8.91 mg/L) 99.99% value was below DPR/EGASPIN MCL. The combination of phytoremediation and vermiremediation techniques in polyculture reactors showed better and spectacular results as the biotas demonstrated good potentiality of hyperbioaccumulation to serve as hydrocarbon sinks from the ecotoxics of TPHs.</p> C. Fawole S. J. Salami D. A. Dashak A. C. Harmony ##submission.copyrightStatement## 2020-10-14 2020-10-14 1 12 10.9734/jabb/2020/v23i830169 Bioremediation of Crude Oil Contaminated Soil Using Pig Droppings and Bone Char https://journaljabb.com/index.php/JABB/article/view/30170 <p>Oil extraction operations as well as equipment failure and infrastructure vandalism have caused serious environmental pollution with crude oil spills world-wide. The remediation of the polluted sites is an environmental problem beckoning for solution. In this study, the possibility of pig droppings and pig bone char mixture (biostimulant) to stimulate and optimize crude oil biodegradation in soil was investigated. Exactly 500g of loamy soil was spiked with 3% (w/w) of crude oil. The spiked soil was amended with varying percentage mixtures of the biostimulant and labelled A – E. The spiked soil without biostimulant served as the Control. Each experiment was setup in six (6) replicates, carried out for six weeks, and destructively sampled and analysed on a weekly basis. The removal efficiencies of the biostimulated and unbiostimulated soils were observed to range from 66.70 to 86.70% and 3.69%, respectively. The biodegradation first-order rate constants ranged from 0.1978 to 0.3391wk<sup>-1</sup> and 0.0050wk<sup>-1</sup> for the biostimulated and unbiostimulated soils, respectively. Optimum removal of total petroleum hydrocarbon (TPH) was observed for biostimulated soil C comprising 50% bone char and 50% pig droppings. Results from biostimulated soils A, B, D and E indicated that pig droppings is a more effective biostimulant than pig bone char. A first-order kinetic model adequately predicted the removal of TPH with the optimum biostimulant. It is concluded that using agro-organic waste materials such as pig droppings and pig bone char in a ratio of 1:1 can offer a simple, effective, inexpensive and environmentally friendly solution to the problem of soil contamination with crude oil.</p> Ejikeme Ugwoha Victor Emeka Amah Gabriel Obiosabofu Oweh ##submission.copyrightStatement## 2020-10-23 2020-10-23 13 24 10.9734/jabb/2020/v23i830170 Microbial Assessment of Grey Water Samples Treated with Activated Carbon Forms of Selected Agro-wastes https://journaljabb.com/index.php/JABB/article/view/30171 <p>This study evaluated the efficacy of activated carbon from rice husk, corn cob and coconut husk wastes in the reduction of microbial properties of grey water samples harvested from students’ hostels. Microscopic characterization, enumerations and identification of microbial isolates were carried out to determine the microbial community before and after the treatment with activated carbon. &nbsp;<em>Staphylococcus</em> sp, <em>Micrococcus </em>sp, <em>Bacillus </em>sp, <em>Salmonella </em>sp, <em>Saccharomyces </em>sp and <em>Penicillium </em>sp were observed to be present in the grey water. Before treatment, Total Heterotrophic Count (THC) was 1.2 x 10<sup>11</sup>cfu/ml, Total Coliform Count (TCC), 6.4 x 10<sup>6</sup>cfu/ml and Total Fungi Count (TFC) 2.2 x 10<sup>10</sup>cfu/ml. THC after the treatment ranged from 1.69 x 10<sup>9 </sup>- 7.6 x 10<sup>10</sup>cfu/ml; TCC, 2.2 x 10<sup>5 </sup>- 7.3 x 10<sup>8</sup>cfu/ml and TFC 1.0 x 10<sup>8</sup> - 1.2 x 10<sup>9</sup>cfu/ml. Reduction in the microbial load after treatment revealed that activated carbons from rice husk, corn cob and coconut husk can be used singly or in combined states for the treatment of wastewater.</p> Nduka Chidimma Adamma Okereke Josephat Nwabueze Peter, Chukwudi ##submission.copyrightStatement## 2020-10-26 2020-10-26 25 35 10.9734/jabb/2020/v23i830171