Journal of Advances in Biology & Biotechnology <p style="text-align: justify;"><strong>Journal of Advances in Biology &amp; Biotechnology (ISSN:&nbsp;2394-1081)</strong> aims to publish high quality papers (<a href="/index.php/JABB/general-guideline-for-authors">Click here for Types of paper</a>) in all areas of ‘Biology &amp; Biotechnology’. By not excluding papers on the basis of novelty, this journal facilitates the research and wishes to publish papers as long as they are technically correct and scientifically motivated. The journal also encourages the submission of useful reports of negative results. This is a quality controlled,&nbsp;OPEN&nbsp;peer reviewed, open access INTERNATIONAL journal.</p> SCIENCEDOMAIN international en-US Journal of Advances in Biology & Biotechnology 2394-1081 Morphological and Molecular Identification of Mycobiota Associated with Juniperus monosperma (Engelm.) Sarg. Blight in Mexico <p>The aim of the investigation was to identify the agents associated with blight in <em>Juniperus monosperma</em> at Lirios region, Arteaga, Coahuila. Botanical material was collected at Lirios, Arteaga, and taken to the laboratory. Pathogens were isolated in ADP culture medium and identifying by morphological criteria and molecular using primers ITS1 and ITS4. DNA extraction by the Dellaporta method, the visualization of obtained DNA was performed by electrophoresis on a 2% (p/v) agarose gel. DNA quantification was performed on a NanoDrop 1000 spectrophotometer, and DNA amplification was carried out in the Veriti thermocycler. Obtained sequences were aligned and compared with those available in the GenBank database of the National Center for Biotechnology Information (NCBI), using the BLAST algorithm (Basic Local Aligment Search Tool) to find conserved sequences. Pathogens were inoculated in stems of <em>J. monosperma</em>. The agents associated with blight were <em>Alternaria</em> sp., <em>Aspergillus</em> sp. and <em>Rosellinia</em> sp. and the sequence obtained compared with BLAST coincided only with <em>Alternaria</em> sp. and <em>Aspergillus</em> sp. with the access codes KP027305.1 and MG551283.1, respectively. According to data taken in field, <em>Alternaria</em> sp. behaved as the highest prevalence species and severity associated with blight in <em>J. monosperma</em>, and <em>Rosellinia</em> sp. in laboratory conditions.</p> Ma. E. Galindo-Cepeda A. Sánchez-Arizpe E. Castro-del Ángel S. F. Velázquez-Morales J. L. Arispe-Vázquez ##submission.copyrightStatement## 2020-12-30 2020-12-30 1 7 10.9734/jabb/2020/v23i1030180 Research Progress on Auricularia delicata <p><strong>Aims:</strong> To describe the current status of <em>A. delicata </em>emphasizing on the key parameters; occurrence, classification, molecular studies, nutritional and medicinal benefits, cultivation status, and future development perspective.</p> <p><strong>Place and Duration of Study: </strong>China–Zambia Agricultural demonstration center and Engineering Research Center of Chinese Ministry of Education for Edible and Medicinal Fungi, Jilin Agricultural University, China between July 2019 and June 2020.</p> <p><strong>Methodology:</strong> In this study, various literatures were reviewed for each parameter studied. Findings were deduced from current literatures and discussed.</p> <p><strong>Results:</strong> The screening of bioactive contents of <em>A. delicata</em> revealed the presence of phenolic compounds; chlorogenic, flavonoids, and ethyl acetate, polysaccharides; Chitosan, fibers, β-glucans, mannans, chitin, and melanin. These substances exhibit hepatoprotective effects, antioxidant activities and antimicrobial activities against some microbes like<em> Candida albicans, Bacillus subtilis, Enterococcus</em> <em>faecium, Streptococcus aureus, Bacillus cereus, Salmonella typhi and Escherichia coli.</em> <em>A. delicata</em> also contains several nutrients namely; protein, vitamin B2, vitamin C, and minerals; Potassium, Calcium, Iron, Magnesium, Zinc, Manganese, that play a vital role in human growth and development. Moreover, its cultivation using various technologies provides an opportunity for high yield production.<em> A. delicata</em> was recently studied at Jilin Agricultural University and domesticated in Heilongjiang province in the northeastern part of China. Due to the similarity of its fruiting body with the structure of the deer tripe, it was assigned a common name as “Deer tripe mushroom”. <em>A. delicata</em> mycelia are capable of growing on several culture media with different nutritional profiles, optimal temperatures and pH values. It is cultivated under tropic temperatures ranging from 25°C-30°C, optimal pH of 6.5, and humidity 80-90%. The commonly used media include; Potato Dextrose Agar (PDA), Yeast Extract Agar (YEA) and Malt Extract Agar (MEA).</p> <p><strong>Conclusion:</strong> Therefore the above mentioned significant properties (occurrence, nutritional, medicinal, and cultivation) provide a foundation for further research on the development and utilization of<em> A. delicata.</em></p> Li Xiao Muharagi Samwel Jacob Zhang Bo Xu Anran ##submission.copyrightStatement## 2020-12-30 2020-12-30 8 32 10.9734/jabb/2020/v23i1030181