Phenol Profiling of Brinjal (Solanum melongena L.) Leaves by LC-MS
B. P. Chauhan *
Department of Agricultural Biotechnology, Anand Agricultural University, Anand- 388 110, Gujarat, India.
Urja B. Solanki
Department of Biochemistry, Anand Agricultural University, Anand, Gujarat-388 110, India.
Y. M. Shukla
Department of Biochemistry, Anand Agricultural University, Anand, Gujarat-388 110, India.
A. A. Sakure
Department of Agricultural Biotechnology, Anand Agricultural University, Anand- 388 110, Gujarat, India.
*Author to whom correspondence should be addressed.
Abstract
Lab experiment was carried out at Department of Agricultural Biotechnology of Anand agricultural university, Anand, Gujarat during 2023-24. Study on phenol profiling was carried out by Liquid Chromatography Mass spectrometry (LC-MS) method. Experimental materials for phenol profiling, fresh leaves of 28 genotypes of brinjal along with parents AB 15-06 (P1) and GRB 5 (P2) selected after three months of transplanting. Total 20 phenolic acids used as standard were; salicylic acid, gallic acid, hydroquinone, esculin hydrate, pyrocatechol, methylumbelliferone, umbelliferon, quercetin, coumaric acid, caffeic acid, chlorogenic acid, ferulic acid, cinnamic acid, syringaldehyde, fraxetin, 4-hydroxy cinnamaldehyde, aminobenzoic acid, catechin hydrate, sinapic acid and epigallocatechin gallate. Out of Twenty, seven (ferrulic aicd, caffeic acid, epigallocatechin gallate, quercetin, salicylic acid, syringaldehyde, chlorogenic acid) phenolic compounds were found to be present in detectable quantities in brinjal leaves.
Keywords: Brinjal, leaves, phenolic acids, LC-MS