Characterization and Determination of Antimicrobial Sensitivity Pattern of Staphylococcus aureus Associated with Urinary Tract Infection
M. Ali *
Department of Microbiology, Kano University of Science and Technology, Wudil, Kano State, Nigeria
S. U. Diso
Department of Pharmaceutical Technology, School of Technology, Kano State, Nigeria
A. U. Zage
Department of Microbiology, Kano University of Science and Technology, Wudil, Kano State, Nigeria.
A. A. Muhammad
Department of Microbiology, Kano University of Science and Technology, Wudil, Kano State, Nigeria
M. Garba
Department of Microbiology, Kano University of Science and Technology, Wudil, Kano State, Nigeria
*Author to whom correspondence should be addressed.
Abstract
Aim: The aim of this study is to characterize and determine the antimicrobial susceptibility pattern of Staphylococcus aureus isolated from urine samples of urinary tract infection patients.
Place and Duration of Study: A total of 20 urine samples were collected from Murtala Muhammad specialist hospital Kano Nigeria, from September to November 2015.
Methodology: Using Gram staining, biochemical characterization and bacteriological method, 16 isolates were confirmed as S. aureus from the 20 different urine samples.
Results: All the 16 isolates were able to ferment Mannitol, showed Golden yellow coloration on Nutrient agar and produce β-haemolysis on blood agar. They also found to be positive for both Catalase and Coagulase test. On sensitivity, the isolates were found to be resistant to Augmentin, Ofloxacin and Amoxicillin. On the other hand, the isolates were sensitive to Ciprofloxacin, Streptomycin, Erythromycin and Neomycin.
Conclusion: This study showed that S. aureus is one of the most frequent aetiologic agents of urinary tract infection.
Keywords: Staphylococcus aureus, urinary tract infection, resistance, antibiotics, gram staining, sensitivity