Modulation of Antioxidant Properties in Leaves of Betel Vine (Piper betel L.) Influenced by the Fungicide Blitox
Poulami Sil
Centurion University of Technology and Management, Odisha 761211, India.
Arunava Chakravarty
Bidhan Chandra Krishi Viswavidyalaya, Mohanpur, Nadia-741252, West Bengal, India.
Shampa Purkaystha *
Centurion University of Technology and Management, Odisha 761211, India.
Punnam Chettri
Centurion University of Technology and Management, Odisha 761211, India.
Goutam Kumar Dash
Department of Biochemistry and Crop Physiology, MS Swaminathan School of Agriculture, Centurion University of Technology and Management, Odisha 761211, India.
Shuvadeep Halder *
Department of Horticulture and Food Science, School of Agriculture and Allied Sciences, The Neotia University, Diamond Harbour, S 24 Pargana-743368, India.
*Author to whom correspondence should be addressed.
Abstract
The experiment was conducted using a Randomized Block Design with three replications at the Plant Virus Research Station, BCKV, Kalyani, in Boroj. On betel vines, Blitox was sprayed at both double the recommended field dose (dRFD) and the recommended field dose (RFD) (50 WP/ha @ 15gm ai/6 lit). The vines were sprayed five more times at 15-day intervals beginning when they were 11.08.15 days old. Before every spray, leaf samples were taken and subjected to a number of criteria for analysis. Based on statistical analysis, the anti-oxidative characteristics improved most significantly when Blitox was administered at twice the recommended field dose (dRFD) (Blitox 50WP/ha @ 30gm ai/6 lit). Higher levels of free and total phenols, together with enhanced activity of enzymes such polyphenol peroxidase (PPO), superoxide dismutase (SOD), and peroxidase (POD), were indicators of this. Furthermore, testing for ferric reducing antioxidant power (FRAP) and diphenyl picryl hydrazyl (DPPH) revealed increased activity levels in the dRFD therapy in comparison to the RFD and control groups. Overall, these results imply that the larger Blitox dosage strengthens the antioxidant characteristics of betel vine leaves, hence enhancing their health and resistance to fungus-related infections.
Keywords: Betel Vine, blitox, antioxidant, fungicide, perennial crop, crop protection