In-vitro Study of Different Fungal Bio-agents against Root-knot Nematode, Meloidogyne incognita

Ramavath Abhi; M.K. Sharma; Devendra Jain; Manisha; B.L. Baheti; CP Nama; Ram Narayan Kumhar; Deepak Kumar

doi:10.9734/jabb/2024/v27i101430

In-vitro Study of Different Fungal Bio-agents against Root-knot Nematode, Meloidogyne incognita

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Published: 2024-09-18

DOI: 10.9734/jabb/2024/v27i101430

Page: 64-70

Issue: 2024 - Volume 27 [Issue 10]

Ramavath Abhi *

Department of Nematology, Rajasthan College of Agriculture, MPUAT, Udaipur, Rajasthan-313001, India.

M.K. Sharma

Department of Nematology, Rajasthan College of Agriculture, MPUAT, Udaipur, Rajasthan-313001, India.

Devendra Jain

Department of MBBT, Rajasthan College of Agriculture, MPUAT, Udaipur, Rajasthan-313001, India.

Manisha

Department of Nematology, Rajasthan College of Agriculture, MPUAT, Udaipur, Rajasthan-313001, India.

B.L. Baheti

Department of Nematology, Rajasthan College of Agriculture, MPUAT, Udaipur, Rajasthan-313001, India.

CP Nama

Department of Nematology, Rajasthan College of Agriculture, MPUAT, Udaipur, Rajasthan-313001, India.

Ram Narayan Kumhar

Department of Nematology, Rajasthan College of Agriculture, MPUAT, Udaipur, Rajasthan-313001, India.

Deepak Kumar

Department of Nematology, Rajasthan College of Agriculture, MPUAT, Udaipur, Rajasthan-313001, India.

*Author to whom correspondence should be addressed.

Abstract

To assess the antagonistic effect of fungal bio-control agents, such as Trichoderma harzianum, T. viride, Purpureocillium lilacinum, Metarhizium anisopliae, and Beauveria bassiana, on the hatching of egg masses of the root-knot nematode, Meloidogyne incognita, investigations were conducted in vitro. In comparison to the control, bio-control agents were tested at 10⁶ and 10⁷ dilutions on M. incognita egg hatching after 24, 48, 72, 96, and 120 hours of exposure. P. lilacinum 10⁷ concentration and T. harzianum 10⁶ concentration (83.90 % and 83.04 %) were found at par and significantly effective on egg hatching inhibition of M. incognita. Among different concentration, P. lilacinum at 10⁶ concentration (85.46 %) gave maximum egg hatching inhibition followed by P. lilacinum at 10⁷ concentration (83.90 %), T. harzianum at 10⁶concentration (83.04 %) and T. virideat 10⁶ concentration (82.00 %) after 120 hrs. M. anisopliae at 10⁷ concentration (69.46 %) was found least effective at different period of exposure. Further studies to be conducted in pot and field conditions to evaluate the efficacy of these bio-agents against root knot nematode, M. incognita.

Keywords: Fungal bio agents, root knot nematode, antagonistic, In vitro, egg hatching

How to Cite

Abhi, Ramavath, M.K. Sharma, Devendra Jain, Manisha, B.L. Baheti, CP Nama, Ram Narayan Kumhar, and Deepak Kumar. 2024. “In-Vitro Study of Different Fungal Bio-Agents Against Root-Knot Nematode, Meloidogyne Incognita”. Journal of Advances in Biology & Biotechnology 27 (10):64-70. https://doi.org/10.9734/jabb/2024/v27i101430.