LC-MS-Based Targeted Metabolite Profiling and Atomic Absorption Spectrophotmeter Based Mineral Analysis of Methanol Extract of Terminalia bellerica (Gaertn.) Leaves
M. Chitra
*
Department of Crop physiology and Biochemistry, Dr. M. S.S .Agricultural College and Research Institute, Thanjavur, (TNAU), Tamil Nadu, India.
P. Sivakumar
Dr .M.S.S .Agricultural College and Research Institute, Thanjavur, (TNAU), Tamil Nadu, India.
G.G.Kavitha Shree
KVK, Pongalur, TNAU, Tamil Nadu, India.
P. Sujatha
Dr .M.S.S .Agricultural College and Research Institute, Thanjavur, (TNAU), Tamil Nadu, India.
R.Uma Sankareswari
Dr .M.S.S .Agricultural College and Research Institute, Thanjavur, (TNAU), Tamil Nadu, India.
*Author to whom correspondence should be addressed.
Abstract
Aim: Natural products in medicinal plants are one of the important sources for modern drug discovery. Plant-based formulations play an important role in management of numerous diseases. To discover the medical potential of natural products, it is important to understand the bioactive compounds present in medicinal plants. Hence the aim of the study is to identify the volatile compounds by LC –MS analysis and minerals by AAS assay present in the leaves of Terminalia bellerica.
Study Design: This study investigates metabolites present in leaves of Terminalia bellerica have been studied by AAS and LC-MS techniques.
Results: The AAS assay showed that the leaves of T. bellerica are particularly rich in iron (260 mg/kg) compared to their zinc, manganese, and copper content. Meanwhile, the LC-MS analysis identified chebulinic acid, chebulagic acid, punicafolin, myricetin, rutin, and galloyl punicalagin as being present in the highest concentrations.
Conclusion: Results highlighted presence of considerable amount of iron and other minerals in methanolic extract of the leaves of T. bellerica, it may be used in the development of health supplements. The results also revealed that bioactive compounds in the methanolic extracts of T. bellerica leave used for drug design and making it safe to human health.
Keywords: Medicinal herbs, Terminalia bellerica leaves, phytoceutics, atomic absorption spectrophotmeter assay, LC-MS assay