Bio-evaluation and Study of Anticancerous, Antibacterial and Antioxidant Properties of Explants of Piper longum in vivo and in vitro
Sudipta Banerjee *
University Department of Biotechnology, Vinoba Bhave University, Hazaribagh, 825301, India
G. R. Pathade
Department of Biotechnology, Fergusson College, Pune, India
M. A. Mallick
University Department of Biotechnology, Vinoba Bhave University, Hazaribagh, 825301, India
*Author to whom correspondence should be addressed.
Abstract
Piper longum is a medicinal plant of great importance. The present work is to compare anticancerous, antibacterial and antioxidant properties of different explants (leaf and stem) of Piper longum in vivo and in vitro. The anticancerous activity was measured in terms of percentage cytotoxicity and the cell line used was leukemic cell line K562. Escherichia coli, Bacillus subtilis and Staphylococcus aureus, bacteria were used for determination of antibacterial activity. Solvent extracts were prepared from leaf and stem explants of Piper longum and their anticancerous, antibacterial and antioxidant activities were evaluated. Antioxidant activity was measured in terms of percentage (%) Total Phenolic Content (TPC) and percentage (%) 2,2– diphenyl 1-2-picryl hydrazy1 (DPPH) radical scavenging capacity. The anticancerous, antibacterial and antioxidant effects were found to be higher for hot extracts than cold extract. Further the results of in vivo explants were better than in vitro explants, in case of anticancerous activity and results of in vitro explants were better than in vivo explants, in case of antibacterial and antioxidant activity.
Keywords: Piper longum, anticancerous activity, antibacterial activity, antioxidant activity, Leukemic cell line K562, TPC, DPPH