Molecular Confirmation for Gall Midge (Biotype 3) Resistance in Phenotypically Resistant Rice Genotypes Using Functional Markers
Anil Kumar
Department of Genetics and Plant Breeding, College of Agriculture, PJT Agricultural University, Rajendranagar, Hyderabad-500 030, Telangana, India.
Y. Hari *
Regional Agricultural Research Station, Professor Jayashankar Telangana Agricultural University, Warangal 506 007, Telangana, India.
J. Suresh
Department of Genetics and Plant Breeding, College of Agriculture, PJT Agricultural University, Rajendranagar, Hyderabad-500 030, Telangana, India.
B. Srinivas
Regional Agricultural Research Station, Professor Jayashankar Telangana Agricultural University, Polasa – 505 529, Jagtial, Telangana, India.
S. Omprakash
Regional Agricultural Research Station, Professor Jayashankar Telangana Agricultural University, Polasa – 505 529, Jagtial, Telangana, India.
C. Narendra Reddy
Regional Agricultural Research Station, Professor Jayashankar Telangana Agricultural University, Polasa – 505 529, Jagtial, Telangana, India.
Saikat Pal
Department of Genetics and Plant Breeding, College of Agriculture, PJT Agricultural University, Rajendranagar, Hyderabad-500 030, Telangana, India.
R. Shravan Kumar
Regional Agricultural Research Station, Professor Jayashankar Telangana Agricultural University, Warangal 506 007, Telangana, India.
B. Edukondalu
Regional Agricultural Research Station, Professor Jayashankar Telangana Agricultural University, Warangal 506 007, Telangana, India.
*Author to whom correspondence should be addressed.
Abstract
This study performed molecular screening of rice genotypes, which showed no gall infestation in field conditions at the Regional Agricultural Research Station (RARS), Jagtial, during rabi, 2021-22. using functional markers such as “gm3del3” for the gm3 gene, “LRR del” for the Gm4 gene, and “PRP” for the Gm8 gene, six genotypes were analyzed. WGL-1145 contained all three resistance genes (gm3, Gm4, and Gm8). Additionally, three genotypes namely WGL-1147, WGL-1127, and Kakai had the Gm4 and Gm8 genes, while RP-5332-54-11-8-2-13 had only Gm8 and IR72476-B-P-9-3-1-1 possessed only gm3. These findings contribute to developing new rice varieties with resistance to gall midge, which can improve crop yields and food security in affected regions. Some promising rice varieties may be used as donors in breeding programs aimed at creating pyramided lines with durable gall midge resistance.
Keywords: Rice, Gall midge resistance, functional markers