Standardization of Sucrose Concentration in Micropropagation of Valuable Aquascape Plant Echinodorus grisebachii
Greeshma Baby *
Department of Floriculture and Landscaping, College of Agriculture, Vellayani, Thiruvananthapuram- 695522, Kerala, India.
M. Rafeekher
Department of Floriculture and Landscaping, College of Agriculture, Vellayani, Thiruvananthapuram- 695522, Kerala, India.
Soni, K.B
Department of Biotechnology, College of Agriculture, Vellayani, Thiruvananthapuram, Kerala, India.
Priya Kumari I
Department of Floriculture and Landscaping, College of Agriculture, Vellayani, Thiruvananthapuram- 695522, Kerala, India.
Reshmi C.R
Department of Floriculture and Landscaping, College of Agriculture, Vellayani, Thiruvananthapuram- 695522, Kerala, India.
Sheena. A
Instructional Farm, College of Agriculture, Vellayani, Thiruvananthapuram, Kerala, India.
Abdul Rahman M
Department of Horticulture, Floriculture and Landscape Architecture, School of Agricultural Sciences, Nagaland University, Medzhiphema, Nagaland, India.
Hasna P M
Department of Floriculture and Landscaping, College of Agriculture, Vellayani, Thiruvananthapuram- 695522, Kerala, India.
*Author to whom correspondence should be addressed.
Abstract
Aims: To standardize the optimum sucrose concentration in shooting and rooting medium in micropropagation of valuable aquascape plant Echinodorus grisebachii.
Study Design: CRD.
Place and Duration of Study: Department of Floriculture and Landscaping, College of Agriculture Vellayani 2022-2024.
Methodology: In this study, eight treatments were evaluated separately for their effectiveness in shooting and rooting medium. For the shooting medium, 2 mgL-1 BAP (6-Benzylaminopurine) and 3 sucrose concentrations (10, 20,30) gL⁻¹ were used in the MS medium and half-strength MS medium. For rooting medium 3.0 mgL⁻¹ IBA (Indole-3-butyric acid), three concentrations of sucrose as in shooting medium were tried.
Results: The result of the study showed that out of 8 treatments tried for shooting medium MS + 2mgL-1 + 30 gL⁻¹ sucrose was best resulted in Shoot initiation (100 %), Minimum days to shoot emergence (6.17), Maximum number of shoots (9.83), Longest shoot (10.33) and maximum number of leaves (31). For rooting medium ½ MS + 3.0 mgL⁻¹ IBA + 30 gL⁻¹ sucrose was best resulted in minimum number of days in root emergence (12.58) and maximum root initiation percentage (99.25).
Conclusion: An effective concentration of sucrose was standardized for Echinodorus grisebachii.
Keywords: Micropropagation, Echinodorus greisebachii, aquascaping, ecology, floriculture