Assessment of Parental Polymorphism between Swarna / Oryza rufipogon IC309814 Derived Mapping Population
Pranay G
ICAR-Indian Institute of Rice Research, Hyderabad-500030, Telangana, India and Indira Gandhi Krishi Vishwa Vidyalaya, Raipur, Chhattisgarh, India.
NS Tomar
Indira Gandhi Krishi Vishwa Vidyalaya, Raipur, Chhattisgarh, India.
Divya Balakrishnan *
ICAR-Indian Institute of Rice Research, Hyderabad-500030, Telangana, India.
Sunil Nair
Indira Gandhi Krishi Vishwa Vidyalaya, Raipur, Chhattisgarh, India.
PK Joshi
Indira Gandhi Krishi Vishwa Vidyalaya, Raipur, Chhattisgarh, India.
Subbarao LV
ICAR-Indian Institute of Rice Research, Hyderabad-500030, Telangana, India.
N Sarla
ICAR-Indian Institute of Rice Research, Hyderabad-500030, Telangana, India.
SV Saiprasad
ICAR-Indian Institute of Rice Research, Hyderabad-500030, Telangana, India.
RM Sundaram
ICAR-Indian Institute of Rice Research, Hyderabad-500030, Telangana, India.
*Author to whom correspondence should be addressed.
Abstract
Detection of molecular markers with parental polymorphism is a pre-requisite for QTL mapping and further identification of genomic regions. PCR based InDel markers tend to have a greater degree to show polymorphism among the parental genotypes for any specific cross. Polymorphic studies were carried out between the two parents Swarna (Cultivated rice variety) and Oryza rufipogon IC309814 (Wild rice accession) using 192 randomly selected InDel markers and 40 gene specific markers for Iron and Zinc covering the entire 12 chromosomes. Results revealed that InDel markers were polymorphic between both the parents and highest percentage of polymorphism observed on chromosome 10 (75%) followed by chromosome 5 (60%) and chromosome 9 (55.6%) and lowest polymorphism percentage was shown in chromosome 3 (8.3%) with an overall 22.4 % polymorphism percentage among all the markers used in this study. Detected polymorphic (SSR) markers were for genotyping the mapping population derived from the cross between Swarna cultivar and Oryza rufipogon to map Quantitative Trait Loci (QTLs) associated with yield and grain quality traits.
Keywords: Swarna, wild rice, parental polymorphism, InDels and biofortification