Phylogenetic Analysis of Bacillus Bacteria from Compost
Ayesha Ameen *
Department of Biotechnology, University of South Asia, Lahore, Pakistan
Jalil Ahmad
Department of Biotechnology, University of South Asia, Lahore, Pakistan
Shahid Raza
Department of Biotechnology, University of South Asia, Lahore, Pakistan
*Author to whom correspondence should be addressed.
Abstract
Many activated regions of gene can be used to identify the particular organism, this is easier by using the technique of 16S ribotyping of rRNA gene. These regions are present on rRNA gene and are conserved, that is the reason to use them for identification purpose. This study was mainly focused on the phylogenetic analysis of reverse sequence of bacillus strains for 16S rRNA gene. It was concluded that the AB1 and AB2 were closely related and showed direct lineage while the E.coli strain and salmonella showed outgroup relation.
Keywords: PCR, gel electrophoresis, 16S reverse sequence, AB1 and AB2