Hybrid Approach for Electrophoresis Pattern of Seed Storage Proteins and Soil Study (Pedology); a Study on Intra Specific Diversity of Stachys inflata Benth
E. Jahandideh *
Department of Basic Science, Islamic Azad University, East Tehran Branch, Tehran, Iran
A. Chehregani Rad
Department of Biology, Laboratory of Plant Cell Biology, Bu-Ali Sina University, Hamedan, Iran
*Author to whom correspondence should be addressed.
Abstract
Aims: For determination of levels and types of intraspecific diversity in the identified groups, electrophoresis studies and soil studies were employed.
Study Design: This study has designed base on accumulation of species and laboratory works.
Place and Duration of Study: Department of biology, plant laboratory of BASU University in Hamedan and the duration was between September 2009 and September 2010.
Methodology: Stachys, a genus of the Lamiaceae family, with more than 300 species is wildly distributed throughout the world. DSS method (Determination of Special Station) is the method of choice for this study which has established itself as an efficient method to study intraspecific diversity. 13 special stations for Stachys inflata were selected where 72 plant species are distinguished as associated species of these special stations. Floristic-ecologic data analyzed by Anaphyto software using F.C.A (Factorielle Correspondance Analysis) method.
Results: Comparison of the results led to determination of 9 groups for this species which reveals the existence of intraspecific diversity in Stachys inflata. The electrophoretic profiles of seed storage proteins are in accordance with floristic- ecologic and soil study groupings. Results obtained from the analysis of electrophoresis of seed storage proteins by MVSP (Multi Variante Statistical Package) and NTSYS software were also in accordance with floristic-ecological groupings. The number of bands and their density varied in this species, indicating their intraspecific diversity in the populations of Stachys inflata Benth.
Conclusion: Therefore, in this species, the grouping by floristic marker was confirmed by electrophoresis as well as soil data. As a result by using D.S.S method, the number of groups and their members were completely identical and utilizing ecological data such as soil caused to detect intraspecific diversity in this species.
Keywords: DSS method, electrophoresis, intraspecific diversity, soil data, Stachys inflate