Comparison of 16S rRNA Gene of Bacillus cereus with Different Bacterial Species
Shahid Raza *
Department of Biotechnology, University of South Asia, Lahore, Pakistan
Ayesha Ameen
Department of Biotechnology, University of South Asia, Lahore, Pakistan
*Author to whom correspondence should be addressed.
Abstract
The bacterial 16S rRNA gene increasingly used to discover the species and strains of unknown bacteria. These small 16S rRNA sequences are highly conserved regions. This study was designed to amplify the 16S rRNA gene of Bacillus cereus and compare it with the Coding DNA Sequences of different bacterial species by phylogenetic analysis. The main objective of this study is to construct a phylogram between B. cereus and other bacterial species. The CDS of these bacterial species were taken from NCBI. It was concluded from this study that the phylogram obtained from the sequences reveals that first three species are in group and closely related while other two species are out group, belongs to same ancestor but do not show very close lineage.
Keywords: 16S rRNA gene, B. cereus, PCR, CDS