Metabolic Profiling of the Oyster Spent Mushroom Substrate (SMS) Extract against Some Soil Borne Fungal Plant Pathogens
Pubali Bezbaruah *
Department of Plant Pathology, Assam Agricultural University, Jorhat 785013, Assam, India.
Supriya Sharma
Department of Plant Pathology, Assam Agricultural University, Jorhat 785013, Assam, India.
Apurba Das
Department of Plant Pathology, Assam Agricultural University, Jorhat 785013, Assam, India.
Gunadhya Kumar Upamanya
Department of Plant Pathology, SCS College of Agriculture, Assam Agricultural University, Dhubri, Assam, India.
Devayani Sarmah
Department of Plant Pathology, Assam Agricultural University, Jorhat 785013, Assam, India.
Darshana Sarmah
Department of Botany, North- Eastern Hill University (NEHU), Umshing Mawkynroh, Shillong 793022, Meghalaya, India.
*Author to whom correspondence should be addressed.
Abstract
Mushroom farming is environment-friendly, utilizing agricultural, poultry, and brewery residues. Spent mushroom substrate (SMS) is a by-product after several mushroom cultivation cycles. SMS may contain beneficial microorganisms with potential antagonistic activity against phytopathogens. The use of oyster mushroom substrates has shown promising results in managing various plant diseases. This study explores the antimicrobial potential of water extracts from spent mushroom substrate (SMS) of various Pleurotus species, a byproduct of oyster mushroom cultivation. LCMS analysis identified several bioactive compounds with known antimicrobial properties: Pleurotus sajor-caju SMS contained 3-(o-chlorophenyl)-5-(ptolyloxymethyl)-2-oxazolidone; Pleurotus sapidus SMS revealed the presence of Cichoriin, Ethiin, and Cafestol palmitate; while Pleurotus ostreatus SMS showed Bronopol and Daphnetin-8-glucoside. These findings demonstrate the presence of natural antimicrobial agents in Oyster mushroom SMS, suggesting its potential as an eco-friendly, sustainable alternative for managing soil-borne fungal plant pathogens. This approach not only supports environmental sustainability but also adds value to agricultural waste through its application in plant disease management.
Keywords: Pleurotus ostreatus, Pleurotus sajorcaju, Pleurotus sapidus, antimicrobial, spent mushroom substrate