Isolation and Identification of Bordetella bronchiseptica from Clinical Respiratory Tract Infections of Canines in Mizoram: First Report
Kh. Thanila Rose
Department of Veterinary Medicine, College of Veterinary Sciences and Animal Husbandry, Central Agricultural University (I), Selesih PO, Aizawl, Mizoram, 796015, India.
J. B. Rajesh
*
Department of Veterinary Medicine, College of Veterinary Sciences and Animal Husbandry, Central Agricultural University (I), Selesih PO, Aizawl, Mizoram, 796015, India.
Sherry Carelyne Marwein
Department of Veterinary Medicine, College of Veterinary Sciences and Animal Husbandry, Central Agricultural University (I), Selesih PO, Aizawl, Mizoram, 796015, India.
Payel Kar
Department of Veterinary Medicine, College of Veterinary Sciences and Animal Husbandry, Central Agricultural University (I), Selesih PO, Aizawl, Mizoram, 796015, India.
Elone Lucy
Department of Veterinary Medicine, College of Veterinary Sciences and Animal Husbandry, Central Agricultural University (I), Selesih PO, Aizawl, Mizoram, 796015, India.
Ankita Debnath
Department of Veterinary Medicine, College of Veterinary Sciences and Animal Husbandry, Central Agricultural University (I), Selesih PO, Aizawl, Mizoram, 796015, India.
Jashima Debbarma
Department of Veterinary Medicine, College of Veterinary Sciences and Animal Husbandry, Central Agricultural University (I), Selesih PO, Aizawl, Mizoram, 796015, India.
Parimal Roychoudhury
Department of Veterinary Microbiology, College of Veterinary Sciences and Animal Husbandry, Central Agricultural University (I), Selesih PO, Aizawl, Mizoram, 796015, India.
T. K. Dutta
Department of Veterinary Microbiology, College of Veterinary Sciences and Animal Husbandry, Central Agricultural University (I), Selesih PO, Aizawl, Mizoram, 796015, India.
T. C. Tolenkhomba
Department of Animal Genetics & Breeding, College of Veterinary Sciences and Animal Husbandry, Central Agricultural University (I), Selesih PO, Aizawl, Mizoram, 796015, India.
*Author to whom correspondence should be addressed.
Abstract
Bordetella bronchiseptica is one of the major bacterial causes of Canine Infectious Respiratory Disease Complex (CIRDC) with zoonotic potential. This research was designed to give information on the detection of B. bronchiseptica in presented canine cases at the Veterinary Clinical Complex. Oro-nasal and pharyngeal swabs were collected from 113 from canine cases suspected for respiratory tract infection. Detection was done by culture, isolation and identification, biochemical test and confirmatory diagnosis by Polymerase Chain Reaction. Luria Bertani broth was used for the pre-enrichment and Smith-Baskerville media were used for isolating B. bronchiseptica. The organism was grown in MacConkey agar and isolated using specific medium (Smith-Baskerville). Isolates on MacConkey agar showed non-lactose fermenting small pale colonies. The isolated organisms were positive for citrate, urease, catalase, and oxidase test and negative for sucrose and glucose fermentation test. The confirmation was done using PCR with published primers targeting fla gene. Samples from 12 canines were positive for the genus specific amplicon size (237 bp) of B. bronchiseptica. As the presence of the agent is confirmed in many research works, immunization of the puppies can be considered.
Keywords: Bordetella bronchiseptica, canine, clinical, PCR, respiratory tract infection