In-vitro Evaluation of Biocontrol Agents against Eggs of Meloidogyne incognita Infesting Mulberry
Kruthika M S *
Department of Sericulture, College of Sericulture, Chintamani, University of Agricultural Sciences, Bangalore, Karnataka, India.
Ramakrishna Naika
Department of Sericulture, College of Sericulture, Chintamani, University of Agricultural Sciences, Bangalore, Karnataka, India.
Mahesh M
Department of Plant Pathology, College of Sericulture, Chintamani, University of Agricultural Sciences, Bangalore, Karnataka, India.
Rakshitha M P
Department of Sericulture, College of Sericulture, Chintamani, University of Agricultural Sciences, Bangalore, Karnataka, India.
Bharathi V P
Department of Sericulture, College of Sericulture, Chintamani, University of Agricultural Sciences, Bangalore, Karnataka, India.
*Author to whom correspondence should be addressed.
Abstract
The present study aimed to assess the in-vitro efficacy of selected biocontrol agents in inhibiting egg hatching of Meloidogyne incognita to evaluate the efficacy of various bioagents viz., Trichoderma harzianum, Paecilomyces lilacinus, Lecanicillium lecanii, Bacillus subtilis, Pseudomonas fluorescens, microbial consortia1 (P. fluorescens+ B. subtilis+ L. lecanii) and microbial consortia2 (T. harzianum +P. fluorescens+ L. lecanii). Experimental results showed that all the bioagents significantly reduced the hatching rate of juveniles and increased the egg hatching inhibition rate. Among the bioagents, microbial consortia 1 (P. fluorescens + B. subtilis + L. lecanii) was found to be the most effective treatment with a minimum juvenile hatching rate of 9.67% and maximum egg hatching inhibition rate of 84.97% at 100% concentration after 72 hours, followed by microbial consortia 2 (T. harzianum + P. fluorescens + L. lecanii) and Paecilomyces lilacinus.
Keywords: Meloidogyne incognita, bioagents, microbial consortia, egg hatching inhibition, mulberry