Genetic Diversity Analysis in Mungbean (Vigna radiata L.) Genotypes Using SSR Markers
MD Afroz *
Regional Agriculture Research Station, Warangal, PJTSAU-506 007, India.
Premakumar
Division of Genetics, ICAR-Indian agricultural research institute, New Delhi, 110012, India.
Imatiyazahamed Teli
Division of Vegetable science, ICAR-Indian agricultural research institute, New Delhi, 110012, India.
Hima Kumar Anakali
Department of Genetics and Plant Breeding, College of Agriculture, Rajendranagar, Hyderabad, India.
V Thirumala Rao
Regional Agriculture Research Station, Warangal, PJTSAU-506 007, India.
Y Hari
Regional Agriculture Research Station, Warangal, PJTSAU-506 007, India.
V Sridhar
Regional Agriculture Research Station, Warangal, PJTSAU-506 007, India.
*Author to whom correspondence should be addressed.
Abstract
Background: Nineteen mungbean [Vigna radiata (L.) Wilczek] genotypes were assessed for molecular diversity using simple sequence repeat (SSR) markers.
Methods: A total of 26 SSR primer pairs were employed to evaluate genetic variation among the genotypes. The amplified products were scored in a binary format, and similarity coefficients were calculated using Jaccard’s method in NTSYS-pc version 2.1. Using the Unweighted Pair Group Method with Arithmetic Mean (UPGMA), a dendrogram was produced.
Results: Among the 26 SSR primers screened, eleven exhibited polymorphism, while thirteen were monomorphic and two did not yield any amplification. Genetic similarity coefficients ranged from 0.22 to 1.00, with an average of 0.61. The genotypes SML-668 and MGG-551 showed complete genetic similarity (1.00), whereas Tarm-18 and GP-2-57 were the most divergent (0.30). These results suggest that SSR markers are effective tools for distinguishing mungbean genotypes and for identifying suitable parental combinations for breeding programs.
Keywords: Vigna radiata, SSR markers, genetic similarity, molecular diversity, UPGMA