Phytochemical Characterization of Herbal Formulation Containing Nigella sativa, Terminalia arjuna, Withania somnifera and Linum usitatissimum

Pankaj Kumar Umar *

Department of Veterinary Pharmacology and Toxicology, College of Veterinary Science (NDVSU), Jabalpur, MP, India.

Vidhi Gautam

Department of Veterinary Pharmacology and Toxicology, College of Veterinary Science (NDVSU), Jabalpur, MP, India.

Jyoti

Department of Veterinary Pharmacology and Toxicology, College of Veterinary Science (NDVSU), Jabalpur, MP, India.

Sachin Jain

Department of Veterinary Pharmacology and Toxicology, College of Veterinary Science (NDVSU), Jabalpur, MP, India.

Shashank Vishvakarma

Department of Veterinary Gynaecology and Obstetrics, College of Veterinary Science (NDVSU), Jabalpur, MP, India.

Anil Gattani

Department of Veterinary Physiology and Biochemistry, College of Veterinary Science (NDVSU), Jabalpur, MP, India.

R.K. Sharma

Department of Veterinary Pharmacology and Toxicology, College of Veterinary Science (NDVSU), Jabalpur, MP, India.

Anand Jain

Department of Veterinary Physiology and Biochemistry, College of Veterinary Science (NDVSU), Jabalpur, MP, India.

*Author to whom correspondence should be addressed.


Abstract

Hyperlipidemia, a metabolic disorder characterized by elevated plasma lipid and lipoprotein levels, is a major risk factor for cardiovascular diseases. Although synthetic drugs such as statins and fibrates are widely used for treatment, their long-term use is often associated with adverse effects, prompting the search for safer alternatives. Medicinal plants rich in bioactive phytoconstituents offer promising therapeutic potential in managing dyslipidemia. The present study was conducted to perform phytochemical screening of ingredients in a herbal formulation comprising Nigella sativa, Terminalia arjuna, Withania somnifera and Linum usitatissimum. The aqueous extract was prepared by cold maceration and analyzed for major phytochemical constituents using standard qualitative tests. Phytochemical analysis revealed the presence of alkaloids, tannins, terpenoids, saponins, phenols, glycosides, flavonoids, and coumarins. Overall, the findings validate the traditional use of these herbal formulations as safe and effective alternatives for the prevention and management of hyperlipidemia.

Keywords: Phytochemical screening, Herbal formulation, Nigella sativa, Terminalia arjuna, Withania somnifera, Linum usitatissimum, hyperlipidemia


How to Cite

Umar, Pankaj Kumar, Vidhi Gautam, Jyoti, Sachin Jain, Shashank Vishvakarma, Anil Gattani, R.K. Sharma, and Anand Jain. 2025. “ Phytochemical Characterization of Herbal Formulation Containing Nigella Sativa, Terminalia Arjuna, Withania Somnifera and Linum Usitatissimum”. Journal of Advances in Biology & Biotechnology 28 (11):957-63. https://doi.org/10.9734/jabb/2025/v28i113290.

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