Phytochemical Screening and Quantitative Estimation of Quercetin and Gallic Acid in Ethanolic Extract of Moringa oleifera Leaves by HPTLC
Jyoti
*
Department of Veterinary Pharmacology and Toxicology, College of Veterinary Science (NDVSU), Jabalpur, MP, India.
R.K. Sharma
Department of Veterinary Pharmacology and Toxicology, College of Veterinary Science (NDVSU), Jabalpur, MP, India.
Pankaj Kumar Umar
Department of Veterinary Pharmacology and Toxicology, College of Veterinary Science (NDVSU), Jabalpur, MP, India.
Kshemankar Shrman
Department of Veterinary Pharmacology and Toxicology, College of Veterinary Science (NDVSU), Jabalpur, MP, India.
Sachin Jain
Department of Veterinary Pharmacology and Toxicology, College of Veterinary Science (NDVSU), Jabalpur, MP, India.
Vidhi Gautam
Department of Veterinary Pharmacology and Toxicology, College of Veterinary Science (NDVSU), Jabalpur, MP, India.
Anil Gattani
Department of Veterinary Physiology and Biochemistry, College of Veterinary Science (NDVSU), Jabalpur, MP, India.
Shashank Vishvakarma
Department of Veterinary Gynaecology and Obstetrics, College of Veterinary Science (NDVSU), Jabalpur, MP, India.
*Author to whom correspondence should be addressed.
Abstract
Moringa oleifera (family Moringaceae), commonly known as the “Miracle Tree,” is widely recognized for its rich nutritional and therapeutic properties. The present study investigated the phytochemical composition and quantified major antioxidant compounds, quercetin and gallic acid, in ethanolic leaf extract of M. oleifera using High-Performance Thin Layer Chromatography (HPTLC). Preliminary phytochemical screening confirmed the presence of carbohydrates, phenols, tannins, triterpenoids, flavonoids, and alkaloids. Quantitative estimation by HPTLC revealed quercetin and gallic acid contents of 860 µg/g (0.08%) and 273 µg/g (0.03%), respectively. The results validate the antioxidant richness of M. oleifera and support its potential application in pharmaceutical and nutraceutical formulations.
Keywords: Moringa oleifera, HPTLC, phytochemicals, quercetin, gallic acid, antioxidant potential