Comparative Study of Estradiol-Supported In-vitro Maturation in Hariana and Sahiwal Oocytes under Weekly and Bi-Weekly Protocols
Anoop Kumar *
Department of Veterinary Gynaecology and Obstetrics, College of Veterinary Science and Animal Husbandry, U.P. Pandit Deen Dayal Upadhyay Pashu Chikitsa Vigyan Vishwavidyalaya Evam Go Anusandhan Sansthan (DUVASU), Mathura-281001, Uttar Pradesh, India.
Atul Saxena
Department of Veterinary Gynaecology and Obstetrics, College of Veterinary Science and Animal Husbandry, U.P. Pandit Deen Dayal Upadhyay Pashu Chikitsa Vigyan Vishwavidyalaya Evam Go Anusandhan Sansthan (DUVASU), Mathura-281001, Uttar Pradesh, India.
Mukul Anand
Department of Veterinary Physiology, College of Veterinary Science and Animal Husbandry, U.P. Pandit Deen Dayal Upadhyay Pashu Chikitsa Vigyan Vishwavidyalaya Evam Go Anusandhan Sansthan (DUVASU), Mathura-281001, Uttar Pradesh, India.
Ashutosh Mishra
Department of Veterinary Gynaecology and Obstetrics, College of Veterinary Science and Animal Husbandry, U.P. Pandit Deen Dayal Upadhyay Pashu Chikitsa Vigyan Vishwavidyalaya Evam Go Anusandhan Sansthan (DUVASU), Mathura-281001, Uttar Pradesh, India.
Amrita Priyadershi
Department of Veterinary Gynaecology and Obstetrics, College of Veterinary Science and Animal Husbandry, U.P. Pandit Deen Dayal Upadhyay Pashu Chikitsa Vigyan Vishwavidyalaya Evam Go Anusandhan Sansthan (DUVASU), Mathura-281001, Uttar Pradesh, India.
Pratyanshu Srivastava
Department of Veterinary Gynaecology and Obstetrics, College of Veterinary Science and Animal Husbandry, U.P. Pandit Deen Dayal Upadhyay Pashu Chikitsa Vigyan Vishwavidyalaya Evam Go Anusandhan Sansthan (DUVASU), Mathura-281001, Uttar Pradesh, India.
*Author to whom correspondence should be addressed.
Abstract
Estradiol is a key hormone that helps in the development and growth of bovineoocytes, playing an important role in in-vitro maturation (IVM) outcomes for cattle. This study evaluated the effects of estradiol supplementation at concentrations of 0.5 μg/mL, 1.0 μg/mL, and 1.5 μg/mL on the maturation of oocytes retrieved from super-stimulated Hariana and Sahiwal cows (n = 6 per breed) using ovum pick-up (OPU) protocols. Weekly and bi-weekly OPU intervals were compared to assess the influence of retrieval frequency. Maturation rates were evaluated through cumulus expansion and polar body extrusion. Data were analyzed using one-way ANOVA with significance set at 5% and 1%. The results revealed optimal maturation rates of 80.44% at 1.5 μg/mL and 73.88% at 1.0 μg/mL estradiol, respectively, with weekly OPU yielding superior outcomes. These findings provide valuable insights into refining IVM protocols and advancing assisted reproductive technologies (ARTs) for sustainable cattle breeding.
Keywords: Assisted reproductive technologies, bovine oocytes, estradiol, In-vitro maturation, ovum pick-up