Genetic Identification of Soybean Segregants Null for Kunitz Trypsin Inhibitor (KTI) and Lipoxygenase-2 (LOX2)
Shinde C. S. *
SLBTC, Mahatma Phule Krishi Vidyapeeth, Rahuri – 413 722, Ahilyanagar, Maharashtra, India.
Chimote V. P.
SLBTC, Mahatma Phule Krishi Vidyapeeth, Rahuri – 413 722, Ahilyanagar, Maharashtra, India.
Deshmukh M. P.
Agriculture Research Station, Kasbe Digraj – 416 305, Sangli, Maharashtra, India.
*Author to whom correspondence should be addressed.
Abstract
The present investigation entitled was conducted at the Post Graduate Research Farm, Mahatma Phule Krishi Vidyapeeth, Rahuri and State Level Biotechnology Centre, Rahuri – 413 722 during the academic year 2024-25. The objectives included in this study was to depict the molecular markers linked to the quality traits viz., KTI and LOX2 and identify desirable segregants in soybean. A total of 100 F2 segregants 50 each from Cross I (JS 22 12 × NRC 142) and Cross II (JS 22 16 × NRC 142) were evaluated using three quality trait markers viz., titi gene specific was linked to KTI locus while remaining two, lox2 gene specific and Satt 656 linked to Lox-2 gene locus. The markers were used to identify segregants free from KTI and LOX2 genes. Out of 100 F2 segregants, 11 (#3, #5, #6, #8, #9, #15, #17, #20, #22, #38, #41) segregants from Cross I and 7 (#3, #9, #16, #26, #41, #44, #47) segregants from Cross II (JS 22 16 × NRC 142) were identified as free from KTI and LOX2 factors. These identified segregants were subsequently advanced to develop homozygous lines exhibiting null activity for both KTI and LOX2.
Keywords: Segregants, KTI, LOX2, homozygous, Lipoxygenase-2