Effect of Auxin Treatments and Seasonal Variation on Stem Cuttings Propagation of Hildegardia populifolia (DC.) Schott & Endl.

Akkenapally Snehanjali

Department of Forest Biology and Tree Improvement, Forest College and Research Institute Hyderabad at Mulugu, Siddipet-502279, Telangana, India.

Chennuru Nagendra

Department of Botany, Yogi Vemana University, Kadapa 516005, Andhra Pradesh, India.

A. Madhusudhana Reddy

Department of Botany, Yogi Vemana University, Kadapa 516005, Andhra Pradesh, India.

Reeja Sundaram

Department of Forest Biology and Tree Improvement, Forest College and Research Institute Hyderabad at Mulugu, Siddipet-502279, Telangana, India.

Sreedhar Bodiga

Department of Basic and Social Sciences, Forest College and Research Institute, Hyderabad at Mulugu, Siddipet-502279, Telangana, India.

Rachapudi Venkata Sreeharsha

Department of Life Sciences, CSJM University, Kanpur, Uttarpradesh,208024, India.

Shalini Mudalkar *

Department of Forest Biology and Tree Improvement, Forest College and Research Institute Hyderabad at Mulugu, Siddipet-502279, Telangana, India.

*Author to whom correspondence should be addressed.


Abstract

Aims: To evaluate the effect of different concentrations of indole-3-butyric acid (IBA) and naphthalene acetic acid (NAA) on rooting and sprouting of stem cuttings of Hildegardia populifolia during spring and rainy seasons.

Study Design: A field-based greenhouse experiment was carried out under nursery conditions.

Place and Duration of Study: Forest College and Research Institute, Mulugu, Telangana, during 2022-2025 duration of research work.

Methodology: Stem cuttings (30 cm long, 2–3 nodes) were treated with seven auxin-based formulations (IBA 1000, 2000, 3000 ppm; NAA 1000, 2000, 3000 ppm; and control) and planted in polybags containing red soil, black soil and sand (2:1:1) supplemented with cocopeat. Observations on sprouting percentage, rooting percentage, mean number of roots and root length were recorded for both spring and rainy seasons.

Results: IBA at 3000 ppm produced the highest sprouting (40% in spring, 33.33% in rainy), rooting (33% in spring, 26.66% in rainy), number of roots (11.66 in spring, 3.0 in rainy) and root length (12.95 cm in spring, 2.33 cm in rainy). Overall, the spring season recorded significantly higher rooting and growth compared to the rainy season.

Conclusion: IBA at 3000 ppm applied during spring is the most effective treatment for vegetative propagation of Hildegardia populifolia. Seasonal variation strongly influences rooting success, and the spring season is recommended for large-scale nursery multiplication.

Keywords: Hildegardia populifolia, IBA, NAA, stem cuttings, vegetative propagation, seasonal variation


How to Cite

Snehanjali, Akkenapally, Chennuru Nagendra, A. Madhusudhana Reddy, Reeja Sundaram, Sreedhar Bodiga, Rachapudi Venkata Sreeharsha, and Shalini Mudalkar. 2026. “Effect of Auxin Treatments and Seasonal Variation on Stem Cuttings Propagation of Hildegardia Populifolia (DC.) Schott & Endl”. Journal of Advances in Biology & Biotechnology 29 (2):927-35. https://doi.org/10.9734/jabb/2026/v29i23690.

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