Journal of Advances in Biology & Biotechnology

Global agricultural pest management incurs substantial annual expenditures exceeding billions of dollars. For many decades, broad-spectrum synthetic insecticides were regarded as the most effective option for pest suppression. However, their indiscriminate and excessive use has accelerated the evolution of resistance in pest populations, disrupted natural enemies, and posed serious threats to human health and environmental safety. Therefore, sustainable pest management requires minimizing chemical pesticide use and conserving beneficial organisms to maintain ecological balance. Modern biotechnology provides promising and environmentally sound alternatives for pest control and has significantly reshaped present-day agriculture. Techniques such as genetic transformation and genetic engineering for crop protection—including Bt crops, recombinant DNA technology, gene pyramiding, RNA interference (RNAi), and the sterile insect technique—are now widely adopted for eco-friendly pest management. Emerging gene-editing tools, especially CRISPR-Cas9, offer new opportunities for precise control of insect pests. RNA interference strategies utilizing double-stranded RNA enable targeted elimination of pest species while preserving non-target organisms. Likewise, gene-drive systems modify specific gene inheritance patterns, providing effective population management capabilities. The integration of agricultural biotechnology thus provides innovative, sustainable, and effective solutions to rising pest challenges while supporting long-term food security for the growing global population. Insect biotechnology provides valuable assets across diverse industries including the development of novel catalysts, microbial insect toxins, and other substances. It enables genetic characterization and engineering of organisms and cells, along with recombinant DNA technology applications, creating microbial agents with enhanced insecticidal potency.