Experimental Design for Optimization of β-Xylosidase Production by A. fumigatus Isolated from the Atlantic Forest (Brazil)
Fabíola Giovanna Nesello Vieira
Centro de Ciências Exatas e Tecnológicas, Brazil.
Divair Christ
Centro de Ciências Exatas e Tecnológicas, Brazil.
Luciana Graciano
Centro de Ciências Exatas e Tecnológicas, Brazil.
Juliana Moço Corrêa
Centro de Ciências Exatas e Tecnológicas, Brazil.
Marina Kimiko Kadowaki
Centro de Ciências Médicas e Farmacêuticas, Universidade Estadual do Oeste do Paraná, Cascavel, Paraná, Brazil.
José Luis da Conceição Silva
Centro de Ciências Médicas e Farmacêuticas, Universidade Estadual do Oeste do Paraná, Cascavel, Paraná, Brazil.
Rinaldo Ferreira Gandra
Centro de Ciências Médicas e Farmacêuticas, Universidade Estadual do Oeste do Paraná, Cascavel, Paraná, Brazil.
Alexandre Maller
Centro de Ciências Médicas e Farmacêuticas, Universidade Estadual do Oeste do Paraná, Cascavel, Paraná, Brazil.
Maria de Lourdes Teixeira de Moraes Poli
Departamento de Biologia, Universidade de São Paulo, Ribeirão Preto, São Paulo, Brazil.
Rita de Cássia Garcia Simão *
Centro de Ciências Médicas e Farmacêuticas, Universidade Estadual do Oeste do Paraná, Cascavel, Paraná, Brazil.
*Author to whom correspondence should be addressed.
Abstract
The production of β-Xylosidase by a new strain of Aspergillus fumigatus (PC-7S-2 M), isolated from the Brazilian Atlantic Forest, was analyzed at 28°C using modified Czapeck media supplemented with different agroindustrial residues at 1% (w/v). Conidia were inoculated for 7 days, and the best activity for β- Xylosidase was obtained in the presence of barley brewing residue after 4 days (15 U mL-1). To optimize the production of β-Xylosidase, this carbon source was used for a central composite rotational design (CCRD) to obtain a significance level of p < 0.10, which predicted an enzyme activity of 245.04 U mL-1. The model validation revealed β-Xylosidase activity was optimized at 229.06 U mL-1. Furthermore, the production of intracellular A. fumigatus β-Xylosidase increased by 1,500% (15 times) over that initially obtained, achieving 93.47% of the predicted model. This finding obtained during two years emphasizes the availability of A. fumigatus β-Xylosidase production with possible applications in biotechnological processes.
Keywords: Barley brewing residue, experimental design, agroindustrial residue, hemicellulose, β-Xylosidase, Aspergillus fumigatus