Journal of Advances in Biology & Biotechnology

Optimizing the cultivation of Bacillus species is paramount for the scalable production of microbial bio-inputs. This study investigated the application of low-intensity static magnetic fields (100 and 600 Gauss) as a non-invasive physical stimulus to enhance biomass yield in Bacillus amyloliquefaciens and Bacillus subtilis. Standardized inocula (1 × 10⁵ CFU/mL) were cultivated in Müller-Hinton broth under continuous magnetic exposure for 24 hours, with growth dynamics monitored via optical density (OD₄₀₀) and analyzed using Student's t-test (α = 0.05). The results revealed species-specific physiological responses: B. amyloliquefaciens exhibited a robust, intensity-dependent proliferation increase, achieving relative yield gains of 57.85% at 100 Gauss and 90.00% at 600 Gauss compared to the unexposed control (p < 0.05). Conversely, B. subtilis showed only marginal, non-significant growth trends (26.47% and 24.70% at 100 and 600 Gauss, respectively; p > 0.05). These findings suggest that static magnetic fields act as physiological modulators, likely by altering membrane ion flux and accelerating metabolic pathways. Magnetic stimulation represents a promising, cost-effective, and non-invasive strategy for optimizing biomass production in industrial biofactory systems.