Journal of Advances in Biology & Biotechnology

Aims: This study evaluated the individual and interactive effects of applied biostimulants on the growth, yield, quality, and economic performance of radish (Raphanus sativus L.) cv. Pusa Desi.

Place and Duration of Study: The experiment was conducted at the Agricultural Research Farm, Suresh Gyan Vihar University, Jaipur (Rajasthan), during 2025-26.

Methodology: The experiment was laid out in a factorial randomised block design with two factors: commercial biostimulants (control, humic acid @ 3%, fulvic acid @ 3%, and salicylic acid @ 50 mg/L) and traditional biostimulants (control, moringa leaf extract @ 3%, vermiwash @ 3%, and cow urine [1:10]). The 16 treatment combinations were replicated three times, and the observed data were analysed using OPSTAT.

Results: Significant differences were observed among the treatments. Among the treatment combinations, humic acid @ 3% and moringa leaf extract @ 3% (T₁×M₁) recorded the maximum plant height (35.5 cm at 45 DAS and 51.6 cm at harvest), root-shoot ratio (1.15 cm at 45 DAS and 1.20 cm at harvest), average leaf area (218.48 cm² at 45 DAS), leaf area index (3.36 at 45 DAS), number of leaves per plant (8.4 at 30 DAS and 12.61 at 45 DAS), chlorophyll a content (2 mg/g at 30 DAS and 2.2 mg/g at 45 DAS), chlorophyll b content (1.17 mg/g at 30 DAS and 1.33 mg/g at 45 DAS), total chlorophyll content (3.23 mg/g at 30 DAS and 3.24 mg/g at 45 DAS), maximum root diameter (5 cm at 45 DAS and 5.7 cm at harvest), average root weight (123 g at 45 DAS and 130 g at harvest), yield per plot (5.2 kg), yield per hectare (433 q/ha), TSS content in roots (4.24 ºBrix at 45 DAS and 4.4 ºBrix at harvest), vitamin C (27.9 mg/day at 45 DAS and 31.6 mg/day at harvest), total phenolic content (2.16 mg GAE/g at 45 DAS and 2.51 mg GAE/g at harvest), beta-carotene (4.12 mg at 45 DAS and 4.16 mg at harvest), and the highest B:C ratio of 3.16.

Conclusion: The results of our research indicated that the application of humic acid @ 3% and moringa leaf extract @ 3% (T₁×M₁) increased the growth, yield, and quality of radish. However, these findings are indicative and require further experimentation across seasons and locations before broader recommendations can be made.