Assessment Various Concentrations of ZnO-Nanoparticles on Micropropagation for Chenopodium quinoa Willd. Plant
Fayza Ruzayq Al Gethami
Department of Biology, Faculty of Applied Science, Umm Al Qura University, Makkah Al Mukaramah, KSA. and Department of Biology, Faculty of Science, Jeddah University, Jeddah, KSA.
Hameda El Sayed Ahmed El Sayed *
Department of Biology, Faculty of Applied Science, Umm Al Qura University, Makkah Al Mukaramah, KSA.
*Author to whom correspondence should be addressed.
Abstract
Assessment influences various concentrations of ZnO-Nanoparticles (ZnO-NPs) on Chenopodium quinoa Willd. Micro-propagation by cotyledonary nodes explants was achieved. We used different concentrations of ZnO-NPs (0.2, 2, 10, 20 mg/l) and used the medium free from ZnO-NPs as control. The results indicated that the presence ZnO-NPs in medium was good effect on germination rate of quinoa seeds at 2 mg/l concentration, and we noticed a density in roots hair and number or length roots of seedling. In addition, the highest responding of explant to ZnO-NPs 93.33, 80.8% were in MS that supplemented 0.0, 2.0 mg/l respectively. Maximum numbers of roots 4.0 were also observed in MS containing 0.0, 2.0 mg/l. Although, there was positive clearly effect on number leaves of shoots, but there was a sudden decline (from 8.43 to 1.0) occurred by increasing ZnO-NPs concentrations from 2.0 to 20.0 mg/L. However, the ZnO-NPs do not effect on length of shoots, where the lengthiest shoot occurred in MS without ZnO-NPs. Regarding rooting shoots, there was significant effect of ZnO-NPs on both percentage of root and root number, where percentage of root reached to 100% in 10.0 mg/l concentration and roots number was 4.80 roots in the same concentration.
Keywords: Nanotechnology, quinoa plant, micropropagation, ZnO-nanoparticles, plant tissue culture, germination rate.