First Detection of Carbapenemases-Producing Acinetobacter baumannii and Acinetobacter nosocomialis in Côte d’Ivoire
Kossia Manzan Karine Gba *
Centre National de Recherche Agronomique, 01BP 1740 Abidjan 01, Côte d’Ivoire.
Rémy Bonnin
EA7361 “Structure, Dynamic, Function and Expression of Broad Spectrum β-lactamases”, Faculty of Medicine, Université Paris Sud, Université Paris Saclay, LabEx Lermit, Le Kremlin-Bicêtre, France, Evolution and Ecology of Resistance to Antibiotics Unit, Institut Pasteur – APHP -Université Paris Sud, Paris, France and French National Reference Center for Antibiotic Resistance: Carbapenemase-Producing Enterobacteriaceae, Le Kremlin-Bicêtre, France.
Innocent Allepo Abe
Laboratoire de Biotechnologie, Université Felix Houphouët Boigny, Agriculture et Valorisation des Ressources Biologiques, UFR Biosciences 22BP 582, Abidjan 22, Côte d’Ivoire.
Eric Joel Tahou
Laboratoire de Biotechnologie, Université Felix Houphouët Boigny, Agriculture et Valorisation des Ressources Biologiques, UFR Biosciences 22BP 582, Abidjan 22, Côte d’Ivoire.
Nicole Prisca Niego Dangui Makaya
Département de Sciences de la Vie, Université Marien Ngouabi, Ecole Normale Supérieure, Brazzaville, République du Congo and Laboratoire National de Santé Publique, B.P: 120, Brazzaville, République du Congo.
Laurent Dortet
EA7361 “Structure, Dynamic, Function and Expression of Broad Spectrum β-lactamases”, Faculty of Medicine, Université Paris Sud, Université Paris Saclay, LabEx Lermit, Le Kremlin-Bicêtre, France, Evolution and Ecology of Resistance to Antibiotics Unit, Institut Pasteur – APHP -Université Paris Sud, Paris, France, French National Reference Center for Antibiotic Resistance: Carbapenemase-Producing Enterobacteriaceae, Le Kremlin-Bicêtre, France and Bacteriology-Hygiene Unit, Publique/Hôpitaux de Paris, Bicêtre Hospital, Le Kremlin-Bicêtre, France.
Nathalie Guessennd
Institut Pasteur, CHU Cocody-01BP 490 Abidjan 01, Côte d’Ivoire.
Simon-Pierre Assanvo N’guetta
Laboratoire de Biotechnologie, Université Felix Houphouët Boigny, Agriculture et Valorisation des Ressources Biologiques, UFR Biosciences 22BP 582, Abidjan 22, Côte d’Ivoire.
Thierry Naas
EA7361 “Structure, Dynamic, Function and Expression of Broad Spectrum β-lactamases”, Faculty of Medicine, Université Paris Sud, Université Paris Saclay, LabEx Lermit, Le Kremlin-Bicêtre, France, Evolution and Ecology of Resistance to Antibiotics Unit, Institut Pasteur – APHP -Université Paris Sud, Paris, France, French National Reference Center for Antibiotic Resistance: Carbapenemase-Producing Enterobacteriaceae, Le Kremlin-Bicêtre, France and Bacteriology-Hygiene Unit, Publique/Hôpitaux de Paris, Bicêtre Hospital, Le Kremlin-Bicêtre, France.
*Author to whom correspondence should be addressed.
Abstract
Carbapenem resistant Acinetobacter baumannii are spreading worldwide and represent a major health threat for immunocompromised patients. In this study, we describe for the first time NDM-producing A. baumannii and Acinetobacter nosocomialis isolates from Côte d’Ivoire, Africa. Four carbapenem-resistant Acinetobacter spp. isolates were isolated from urinary tract infections from three university hospital centers. The antimicrobial susceptibility testing and carbapenemase detection (CarbAcinetoNP) test were performed. The carbapenemases genes were screened by PCR. Resistome, MLST and phylogeny were performed using whole genome sequencing. All isolates were resistant to β-lactams, aminoglycosides and fluroquinolones. The blaNDM-1 and the blaOXA-58 genes were identified in the three A. baumannii isolates whereas A. nosocomialis carried only the blaNDM-1 gene. An amino acid change S83L was detected in GyrA in all A. baumannii isolates explaining fluoroquinolone resistance. MLST analysis revealed that all A. baumannii isolates belonged to the same sequence type, ST-2. The blaNDM-1 and blaOXA-58 genes could not be transferred, suggesting a chromosomal location. This study highlights the emergence of NDM-1- and OXA-58-producing A. baumannii isolates belonging to the pandemic clone ST-2 in Côte d’Ivoire, Africa. Comparison of these three genomes revealed that they are highly related.
Keywords: New Delhi metallo-β-lactamase, Acinetobacter baumannii, Acinetobacter nosocomialis