Detection and Dissemination of Extented-Spectrum Beta-Lactamases Genes (CTX-M-15 and SHV-187) Isolated in Multi-Drug Resistant Uropathogenic Klebsiella pneumoniae and Escherichia coli in Cote D'ivoire
Tahou Eric Joël *
Université Felix Houphouët Boigny, Laboratoire de Biotechnologie, Agriculture et Valorisation des ressources biologiques, UFR Biosciences 22BP 582, Abidjan 22, Côte D’Ivoire.
Ahouty Ahouty Bernardin
Université Jean Lorougnon Guédé, BP 150 Daloa, Côte d’Ivoire.
Gba Kossia Manzan Karine
Centre National de Recherche Agronomique de Côte d’Ivoire, 01BP 1740 Abidjan 01, Côte d’Ivoire.
Guessennd Kouadio Nathalie
Université Felix Houphouët Boigny, Laboratoire de Biotechnologie, Agriculture et Valorisation des ressources biologiques, UFR Biosciences 22BP 582, Abidjan 22, Côte D’Ivoire and Institut Pasteur, CHU Cocody-01BP 490 Abidjan 01, Côte d’Ivoire.
N’guetta Assanvo Simon-Pierre
Université Felix Houphouët Boigny, Laboratoire de Biotechnologie, Agriculture et Valorisation des ressources biologiques, UFR Biosciences 22BP 582, Abidjan 22, Côte D’Ivoire.
*Author to whom correspondence should be addressed.
Abstract
Enterobacteriaceae are ubiquitous commensal bacteria of humans that have become major causative agents of hospital infections. The objective of the study was to characterized the extended-spectrum beta-lactam resistance genes from multi-drug uropathogenic clinical strains in Côte d'Ivoire. Bacterial strains isolated from various biological products were collected from January 2011 to June 2016 at the Observatoire de la résistance des micro-organismes aux anti-infectieux en Côte d'ivoire (ORMICI). Two hundred and sixty six (266) enterobacterial strains were collected during this study. Antibacterial susceptibility and the presence of bla genes were determined by the solid-state diffusion method and by PCR, respectively. The presence of ESBL was confirmed by the double disc synergy test method and Sequencing was performed. Of the strains collected, the most isolated were Escherichia coli 53 (39.25%) and Klebsiella pneumoniae 36 (26.66%). Antibiotic resistance of more than 50% was observed for gentamicin, norfloxacin, third generation cephalosporins and tobramicin in E coli. Only imipenem had a low resistance rate of 5.6%. However, apart from norfloxacin, the Klebsiella pneumoniae strains tested expressed resistance to aztreonam and second generation cephalosporins in excess of 50%. The rate of resistance to aztreonam and cefotaxin was statistically different between E. coli and Klebsiella pneumoniae strains (p-value ₌ 0,008 and p-value ₌ 0,032 respectively). The genes blaCTX-M, blaSHV, blaTEM, qnr B and a class I integron were detected. After sequencing, the SHV-1, SHV-28, SHV-187 and CTX-M15 variants were detected. Although these results are of low proportions, this may be considered critical for the future, hence the need for a better antibiotic surveillance strategy in Abidjan.
Keywords: Uropathogens, antibiotic, enterobacteriaceae, multidrug-resistant, Côte d’Ivoire
How to Cite
Downloads
References
Ferech M, Coenen S, Malhotra-Kumar S, Dvorakova K, Hendrickx E, Suetens C, et al. European Surveillance of Antimicrobial Consumption (ESAC): outpatient antibiotic use in Europe. J Antimicrob Chemother. 2006;58(2):401‑7.
Organization WH. Antimicrobial resistance global report on surveillance: 2014 summary. World Health Organization; 2014.
Bradford PA. What’s New in beta-lactamases? Curr Infect Dis Rep. févr 2001;3(1):13‑9.
Bush K. Extended-spectrum beta-lactamases in North America, 1987-2006. Clin Microbiol Infect Off Publ Eur Soc Clin Microbiol Infect Dis. janv 2008;14 Suppl 1:134‑43.
Miriagou V, Carattoli A, Fanning S. Antimicrobial resistance islands: resistance gene clusters in Salmonella chromosome and plasmids. Microbes Infect. 1 juin 2006;8(7):1923‑30.
Drieux L. Succès plasmidique: Transmission inter-espèce d’un plasmide portant un gène de métallo-bëta-lactamase [Microbiologie]. [France]: Paris Sud; 2012.
Partridge SR, Tsafnat G, Coiera E, Iredell JR. Gene cassettes and cassette arrays in mobile resistance integrons. FEMS Microbiol Rev. juill 2009;33(4):757‑ 84.
Saladin M, Cao VTB, Lambert T, Donay JL, Herrmann JL, Ould-Hocine Z, et al. Diversity of CTX-M beta-lactamases and their promoter regions from Enterobacteriaceae isolated in three Parisian hospitals. FEMS Microbiol Lett. 9 avr 2002;209(2):161‑8.
EUCAST-CASFM. European Committee on Antimicrobial Susceptibility Testing- Comité de l’Antibiogramme de la Société Française de Microbiologie [Internet]. 2016 [cité 9 sept 2022]. Disponible sur: Available:https://www.eucast.org/
Ben Said L, Jouini A, Klibi N, Dziri R, Alonso CA, Boudabous A, et al. Detection of extended-spectrum beta-lactamase (ESBL)-producing Enterobacteriaceae in vegetables, soil and water of the farm environment in Tunisia. Int J Food Microbiol. 16 juin 2015;203:86‑92.
Krauland MG, Marsh JW, Paterson DL, Harrison LH. Integron-mediated multidrug resistance in a global collection of nontyphoidal Salmonella enterica isolates. Emerg Infect Dis. Mars 2009;15(3):388‑96.
Bao L, Peng R, Ren X, Ma R, Li J, Wang Y. Analysis of some common pathogens and their drug resistance to antibiotics. Pak J Med Sci. 2013;29(1):135‑9.
Rangaiahagari A, Uwizeyimana JP, Nyirabanzi J, Ngoga E, Wane J. Antibiotic sensitivity patterns of enterobacteriaceae isolated at King Faisal Hospital, Kigali - A Three Years Study; 29 juill 2013 [cité 10 sept 2022].
Available:https://tspace.library.utoronto.ca/handle/1807/41177
Hamid HS, Farzaneh EA, Shahrzad T, Mojgan M. The Prevalence Of Antibiotic Resistance Of Enterobacteriaceae Strains Isolated In Community- And Hospital-Acquired Infections In Teaching Hospitals Of Hamadan, West Of Iran. 1 janv 2013;13(1):75‑80.
Raji MA, Jamal W, Ojemhen O, Rotimi VO. Point-surveillance of antibiotic resistance in Enterobacteriaceae isolates from patients in a Lagos Teaching Hospital, Nigeria. J Infect Public Health. Déc 2013;6(6):431‑7.
Abe IA, Koffi M, Sokouri PD, Ahouty BA, N’Djetchi K M, Simaro S, et al. Assessment of drugs pressure on Escherichia coli and Klebsiella spp. uropathogens in patients attending Abobo-Avocatier Hospital, North of Abidjan (Cte dIvoire). Afr J Microbiol Res. 30 Nov 2019;13(29):658‑66.
Cissé B, Zaba F, Méité S, Mlan A, Adoni-Koffi L, Guessend N, et al. Etiologies microbiennes des infections urinaires chez le sujet diabétique au CHU de Yopougon de 2005 à 2007. J Pharm Biol Sci. 15(1):34‑41.
Leotard S, Negrin N. Épidémiologie des entérobactéries sécrétrices de bêta-lactamases à spectre étendu (E-BLSE) au centre hospitalier de Grasse (2005–2008). Pathol Biol. 1 Févr 2010;58(1):35‑8.
Ferjani A, Mkaddemi H, Tilouche S, Marzouk M, Hannechi N, Boughammoura L, et al. Caractéristiques épidémiologiques et bactériologiques des bactéries uropathogènes isolées dans un milieu pédiatrique. Arch Pédiatrie. 1 Févr 2011; 18(2):230‑4.
Cohen R, Raymond J, Faye A, Gillet Y, Grimprel E. Prise en charge des infections urinaires de l’enfant. Recommandations du groupe de pathologie infectieuse pédiatrique de la Société française de pédiatrie et de la Société de pathologie infectieuse de langue française. Arch Pédiatrie. juin 2015;22(6):665‑71.
Belbel Z, Chettibi H, Dekhil M, Ladjama A, Nedjai S, Rolain JM. Outbreak of an armA methyltransferase-producing ST39 Klebsiella pneumoniae clone in a pediatric Algerian Hospital. Microb Drug Resist Larchmt N. août 2014;20(4):310‑5.
Hou X hua, Song X yu, Ma X bo, Zhang S yang, Zhang J qin. Molecular characterization of multidrug-resistant Klebsiella pneumoniae isolates. Braz J Microbiol. 1 Juill 2015;46(3):759‑68.
Hagel S, Stallmach A, Keller P, Pletz M. [Multiresistant Organisms]. Zentralbl Chir. 1 août 2015;140(4):417‑25.
Skurnik D, Andremont A. Antibiothérapie sélectionnante. De la théorie à la pratique. Réanimation. 1 Juin 2006;15(3):198‑204.
Loot C, Nivina A, Cury J, Escudero JA, Ducos-Galand M, Bikard D, et al. Differences in Integron Cassette Excision Dynamics Shape a Trade-Off between Evolvability and Genetic Capacitance. mBio. 28 mars 2017;8(2):e02296-16.
Derakhshan S, Najar Peerayeh S, Fallah F, Bakhshi B, Rahbar M, Ashrafi A. Detection of Class 1, 2, and 3 Integrons Among Klebsiella pneumoniae Isolated from Children in Tehran Hospitals. Arch Pediatr Infect Dis. 15 Juill 2013;1(4):164‑8.
Jones LA, McIver CJ, Rawlinson WD, White PA. Polymerase chain reaction screening for integrons can be used to complement resistance surveillance programs. Commun Dis Intell Q Rep. 2003;27 Suppl:S103-110.
Rowe-Magnus DA, Mazel D. Integrons: natural tools for bacterial genome evolution. Curr Opin Microbiol. 1 oct 2001;4(5):565‑9.
Eslami G, Seyedjavadi SS, Goudarzi H, Fallah F, Goudarzi M. Distribution of Integrons among Multidrug Resistant E. coli and Klebsiella Strains. Res Med. 10 Mars 2010;34(1):61‑5.
Gillings MR. Integrons: Past, present, and future. Microbiol Mol Biol Rev MMBR. Juin 2014;78(2):257‑77.
Cambray G, Guerout AM, Mazel D. Integrons. Annu Rev Genet. 2010; 44:141‑66.
Villegas MV, Kattan JN, Quinteros MG, Casellas JM. Prevalence of extended-spectrum beta-lactamases in South America. Clin Microbiol Infect Off Publ Eur Soc Clin Microbiol Infect Dis. janv 2008;14 Suppl 1:154‑8.
Dallenne C, Da Costa A, Decré D, Favier C, Arlet G. Development of a set of multiplex PCR assays for the detection of genes encoding important beta-lactamases in enterobacteriaceae. J Antimicrob Chemother. Mars 2010;65(3):490‑5.
Storberg V. ESBL-producing enterobacteriaceae in Africa – a non-systematic literature review of research published 2008–2012. Infect Ecol Epidemiol. 1 Janv 2014;4(1):20342.
Guessennd N, Gbonon V, Ouattara N D, Kacou N’douba A, Ekaza E, Yapi D, et al. Prévalence et profile de résistance des entérobactéries productrices de β-lactamases à spectre élargi (BLSE) à Abidjan de 2005 à 2006. Rev Bio-Afr. 2011;(9):5‑11.
Bonnet R. Growing group of extended-spectrum β-Lactamases: the CTX-M enzymes. Antimicrob Agents Chemother. Janv 2004;48(1):1‑14.
Valentin L, Sharp H, Hille K, Seibt U, Fischer J, Pfeifer Y, et al. Subgrouping of ESBL-producing Escherichia coli from animal and human sources: An approach to quantify the distribution of ESBL types between different reservoirs. Int J Med Microbiol. 1 Oct 2014;304(7):805‑16.
Maciuca IE, Williams NJ, Tuchilus C, Dorneanu O, Guguianu E, Carp-Carare C, et al. High Prevalence of Escherichia coli-producing CTX-M-15 Extended-Spectrum Beta-Lactamases in poultry and human clinical isolates in Romania. Microb Drug Resist Larchmt N. Déc 2015;21(6):651‑62.
Breurec S, Guessennd N, Timinouni M, Le T a. H, Cao V, Ngandjio A, et al. Klebsiella pneumoniae resistant to third-generation cephalosporins in five African and two Vietnamese major towns: multiclonal population structure with two major international clonal groups, CG15 and CG258. Clin Microbiol Infect Off Publ Eur Soc Clin Microbiol Infect Dis. avr 2013;19(4):349‑55.
Amana MD, Jacob ZK, Boukaré Z, Rasmata T, Jihad M, Marie B, et al. First detection of shv-type extended spectrum β-lactamases in The University Hospital Complex Paediatric Charles De Gaulle (CHUP-CDG) of Ouagadougou in Burkina Faso. J Asian Sci Res. 15 mai 2014; 4(5):214‑21.
Jemima SA, Verghese S. SHV-28, an extended-spectrum beta-lactamase produced by a clinical isolate of Klebsiella pneumoniae in South India. Indian J Med Microbiol. mars 2009; 27(1):51‑4.