Investigation of Bioaerosols and the Microbiological Indoor Air Quality in an Urban Nursery School in Port Harcourt, Nigeria

Ughala Ezinwanne *

Department of Microbiology, Faculty of Science, University of Port Harcourt, Choba, Port Harcourt, Rivers State, Nigeria.

Okoro, Chisom Loveth

Department of Microbiology, Faculty of Science, University of Port Harcourt, Choba, Port Harcourt, Rivers State, Nigeria.

*Author to whom correspondence should be addressed.


Abstract

Aims: This study was aimed at investigating the microbial quality of air within the confines of nursery school children. Early exposure to these indoor pollutants can lead to major public health concerns which include acute respiratory tract infections, allergies as well as cancer.

Study Design: Random sampling approach was used in the collection of the samples. Air samples were collected from two different classrooms in a nursery school.

Place and Duration of Study: Air samples were collected within the confines of the nursery section of the Demonstration Primary School in the University of Port Harcourt, Rivers State, Nigeria every other weekday in the month of May 2022.

Methodology: Culture media were placed at the four corners of two classrooms in the nursery section. This nursery class comprised of children between the ages of 4-5 years Nutrient, MacConkey and potato dextrose agar media were used to culture airborne microorganisms during the study. For differential identification of bacteria, citrate, motility, oxidase, indole, catalase, methyl red Voges Proskauer, triple salt iron agar, sugar fermentation tests were carried out.

Results: Both bacterial and fungal species of medical importance, such as Bacillus, Shigella, Micrococcus, Serratia, Proteus, Yersinia, Enterobacter, Penicilium, Aspergillus, Candida, Microsporum. Exophiala and Mucor spp were isolated in this study. The most predominant bacterial species among the isolates in the study was Bacillus sp. with the percentage occurrence of 25%. Shigella and Yersinia species had the percentage occurrence of 16.67% respectively other species like Serratia, Micrococcus, Enterobacter and Proteus each had percentage occurrence of 8.33% which was the lowest occurrence. All the fungal isolates had similar or equal percentage occurrence (16.67%).

Conclusion: Exposure to microbial aerosols in nursery schools can lead to several health complications. Thus, recognition, control and monitoring of air quality in schools are crucial in limiting the spread of airborne pathogens.

Keywords: Bioaerosols, microorganisms, environment, children, bacteria, fungi


How to Cite

Ezinwanne , U., & Loveth , O. C. (2023). Investigation of Bioaerosols and the Microbiological Indoor Air Quality in an Urban Nursery School in Port Harcourt, Nigeria. Journal of Advances in Biology & Biotechnology, 26(3), 1–10. https://doi.org/10.9734/jabb/2023/v26i3622

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References

Walser RD, Garvert DW, Karlin BE, Trockel M, Ryu DM, Taylor CB. Effectiveness of Acceptance and Commitment Therapy in treating depression and suicidal ideation in veterans. Behav Res Ther. 2015;74: 25-31.

Vornanen-Winqvist C, Järvi K, Andersson MA, Duchaine C, Létourneau V, Kedves O et al. Exposure to indoor air contaminants in school buildings with and without reported indoor air quality problems. Environ Int. 2020;141:105781.

Ruggieri S, Longo V, Perrino C, Canepari S, Drago G, L’Abbate L et al. Indoor air quality in schools of a highly polluted south Mediterranean area. Indoor Air. 2019;29 (2):276-90.

Yassin MF, Pillai AM. Monitoring of volatile organic compounds in different schools: A determinant of the indoor air quality. Int J Environ Sci Technol. 2019;16(6):2733-44.

Bennett J, Davy P, Trompetter B, Wang Y, Pierse N, Boulic M et al. Sources of indoor air pollution at a New Zealand urban primary school; a case study. Atmos Pollut Res. 2019;10(2):435-44.

Pearson C, Littlewood E, Douglas P, Robertson S, Gant TW, Hansell AL. Exposures and health outcomes in relation to bioaerosol emissions from composting facilities: A systematic review of occupational and community studies. J Toxicol Environ Health B Crit Rev. 2015;18(1):43-69.

Ashmore MR, Dimitroulopoulou C. Personal exposure of children to air pollution. Atmos Environ. 2009;43(1):128-41.

Lee JH, Kim YJ, Kim SH. Indoor air quality and respiratory symptoms in a daycare center. Environ Res. 2018;161:536-43.

Witchmann J, Lin T, Nilsson MAM, Bellander T. PM2.5, soot and NO2 indoor outdoor relationships at homes, Preschools and schools in Stockholm, Sweden. Atmos Environ. 2017;148:182-9.

Kotzias D. Indoor air and human exposure assessment needs and approaches. Exp Toxicol Pathol. 2005;57;Suppl 1:5-7.

Srikanth P, Sudharsanam S, Steinberg R. Bioaerosols in indoor environment: composition, health effects and analysis. Indian J Med Microbiol. 2008;26(4):302-12.

Samuel FH, Abayneh MM. Microbiological quality of indoor air in university libraries. Asian Pac J Trop Med. 2014;4(1):312-7.

Frankel M, Bekö G, Timm M, Gustavsen S, Hansen EW, Madsen AM. Seasonal variations of indoor microbial exposures and their relation to temperature, relative humidity, and air exchange rate. Appl Environ Microbiol. 2012;78(23):8289-97.

Viegas C, Viegas S, Gomes A, Taubel M, Sabino R. Indoor microbial aerosol and its health effects: microbial exposure in public buildings- viruses, bacteria and fungi. J Health Res Sci. 2017:237-52.

Gatchalian ML. An in-depth analysis of the entrepreneurship education in the Philippines: an initiative towards the development of a framework for a professional teaching competency programme for entrepreneurship educators. Int J Res Rev. 2010;5:51-73.

Selman M, Lacasse Y, Pardo A, Carmier Y. Hypersensitity Pneumonitis caused by Fungi. Proc Am Thorac Soc. 2009;7(3).

Reboux G, Piarroux R, Mauny F, Madroszyk A, Millon L, Bardonnet K et al. Role of molds in farmer’s lung disease in Eastern France. Am J Respir Crit Care Med. 2001;163(7):1534-9.

Ostro B, Lipsett M, Mann J, Braxton-Owens H, White M. Air pollution and exacerbation of asthma in African-American children in los Angeles. Epidemiology. 2001;12(2):200-8.

McCormick A, Loeffler J, Ebel F. Aspergillus fumigatus: Contours of an Opportunistic Human Pathogen. Cell Microbiol. 2010;12(11):1535-43.

Abel HAA, Hanan AM. Sedimentation with the Omeliansky formula as an accepted technique for qualifying airborne fungi. Pol J Environ Stud. 2012;21(6):1539-41.

Chithra VS, Shiva Nagendra SMS. Chemical and morphological characteristics of indoor and outdoor particulate matter in an urban environment. Atmos Environ. 2013;77:579-87.

Madureira J, Paciência I, Pereira C, Teixeira JP, Fernandes EDO. Indoor air quality in Portuguese schools: levels and sources of pollutants. Indoor Air. 2015: 1-12.

Salonen H, Duchaine C, Mazaheri M, Clifford S, Lappalainen S, Reijula K et al. Airborne viable fungi in school environments in different climatic regions–A review. Atmos Environ. 2015;104:186-94.

Madureira J, Paciência I, Pereira C, Teixeira JP, Fernandes EDO. Indoor air quality in Portuguese schools: levels and sources of pollutants. Indoor Air. 2015:1-12.

Javed A, Aamir F, Gohar UF, Mukhtar H, Zia-Ui-Haq M, Alotaibi MO et al. The potential impact of smog spell on Human’s health amid COVID-19 rages. Int J Environ Res Public Health. 2021;18(21): 11408.

Kim H, YJ, Kim SH. Indoor air quality and respiratory symptoms in a daycare center. Environ Res. 2018;161:536-43.

Edward JB. Bacillus cereus, a Volatile Human Pathogen. Chem. Clin Microbiol Rev. 2010;23(2):382-98.

Hans HN, Janos M. Epithelial and human infectious diseases. Epig. Human dis; 2018.

Amemeh YZ, Afshin M, Saeed DA, Ebrahim D, Manochehr A, Ebrahim M et al. Evaluation of bioaerosols type, density and modeling of dispersion in inside and outside of different wards of educational hospitals. Environ Sci Pol Res Int. 2022;29(10):14143-57.

Fernando NL, Fedorak PM. Changes at an activated sludge sewage treatment plant alters the numbers of airborne aerobic microorganisms. Water Res. 2005;39(19): 4597-608.

Korzeniewska E. Emission of bacteria and fungi in the air from wastewater treatment plants–a review. Frontiers in Bioscience-Scholar. 2011;3(2):393-407.