Evaluation of the DNA Fragmentation of the Normozoosperms at the Pasteur Institute of Cote D’ivoire
Marie Florence N’Guessan *
Department of Clinical and Fundamental Biochemistry, Institut Pasteur of Cote d’Ivoire, Cote d’Ivoire.
Maxime Kouadio
Department of Biosciences, Felix Houphouet-Boigny University, Abidjan, Cote d’Ivoire.
Sylvain Beourou
Department of Clinical and Fundamental Biochemistry, Institut Pasteur of Cote d’Ivoire, Cote d’Ivoire.
Souleymane Méité
Department of Clinical and Fundamental Biochemistry, Institut Pasteur of Cote d’Ivoire, Cote d’Ivoire.
N’Gbésso Amos Ekissi
Department of Clinical and Fundamental Biochemistry, Institut Pasteur of Cote d’Ivoire, Cote d’Ivoire and Laboratory of Biotechnology Agriculture and Valorization of Biological Resources, Department Biosciences, University Felix Houphouet-Boigny, Abidjan, Cote d’Ivoire.
Founzegue Coulibaly
Faculty of Agriculture, Department of Fisheries and Agro-Industrial Resources, University of San-Pedro, Cote d’Ivoire.
Allico Joseph Djaman
Department of Clinical and Fundamental Biochemistry, Institut Pasteur of Cote d’Ivoire, Cote d’Ivoire and Department of Biosciences, Felix Houphouet-Boigny University, Abidjan, Cote d’Ivoire.
Mireille Dosso
Department of Clinical and Fundamental Biochemistry, Institut Pasteur of Cote d’Ivoire, Cote d’Ivoire.
*Author to whom correspondence should be addressed.
Abstract
Sperm DNA fragmentation appears to be an important indicator of male fertility and an important parameter for evaluating the positive prognosis of a pregnancy. The objective of our study was to determine the prevalence of DNA fragmentation of normozoosperms received at the Institut Pasteur of Cote d’Ivoire (IPCI).
For this we determined the DNA Fragmentation Index (DFI) of spermatozoa as well as its impact in normozoosperms. The spermogram was carried out according to the criteria of the World Health Organization (WHO) and the integrity of the sperm DNA was verified by the acridine orange test.
Of the 18 normozoosperms samples selected, an average of 20.28 ± 11.22% for the DNA fragmentation index was obtained. About 27.78% of the samples presented a high level of DFI which reflects a poor pregnancy prognosis. Thus, 27.78% of normozoosperms men have abnormal DNA which does not allow these individuals to be able to conceive. This study showed that DNA fragmentation is an important factor in sperm quality.
Keywords: Spermatozoa, DNA, fragmentation, infertility