Antioxidant and Antimicrobial Activity of a Medicinal Mushroom, Ganoderma lucidum
Ş. Canpolat *
Central Research Laboratory Center, Niğde Ömer Halisdemir University, Niğde, Türkiye.
E. Y. Canpolat
Department of Biotechnology, Faculty of Art and Scinece, Niğde Ömer Halisdemir University, Niğde, Turkiye.
*Author to whom correspondence should be addressed.
Abstract
This study aimed to evaluate the antioxidant and antimicrobial activity, along with the determination of major fatty acids by GC-MS and the content of total phenolic compounds of Ganoderma lucidum naturally grown in Türkiye. The antioxidant activity was determined by widely used DPPH radical scavenging method. G. lucidum methanol extract exhibited a 90.81% scavenging activity of DPPH. The content of total phenolic compounds was determined as 235.19 µg GAE/mL by Folin-Ciocalteu’s phenol reagent method. The major fatty acids of G. lucidum were characterized by GC-MS. 2,6-di(t-butyl)-4-hydroxybenzoic acid (RT: 30.021 min), hexadecanoic acid (RT: 36.221 min), 9,12-octadecadienoic acid (RT: 39.949 min), 9-octadecenoic acid (RT: 40.076 min) and octadecanoic acid (RT: 40.601 min) were found to be the predominant fatty acid compounds. The methanol and acetone extracts of G. lucidum were also tested against pathogenic microorganisms via agar disc diffusion method and exhibited various levels of inhibition. The highest antimicrobial activity was detected as 17.67±0.47 mm against Enterococcus faecalis ATCC 29212 strain by acetone extract.
Keywords: Ganoderma lucidum, ; antioxidant, antimicrobial activity, GC-MS