Isolation of Rhizosphere and Phyllosphere Bacteria from Cereal Samples
Chaitra Lakkannavar *
Department of Agricultural Microbiology, University of Agricultural Sciences, Raichur, India.
Mahadeva Swamy
Department of Agricultural Microbiology, University of Agricultural Sciences, Raichur, India.
Yallappa M
Department of Agricultural Microbiology, University of Agricultural Sciences, Raichur, India.
Ramesh Y M
Department of Agronomy, University of Agricultural Sciences, Raichur, India.
Satyanarayan Rao
Department of Agronomy, University of Agricultural Sciences, Raichur, India.
*Author to whom correspondence should be addressed.
Abstract
This study presents the isolation of rhizosphere and phyllosphere bacteria from cereal samples, highlighting their importance in agricultural ecosystems. Employing surface sterilization techniques, selective media and molecular analysis, diverse bacterial communities were identified on the root and leaf surfaces of cereal crops, encompassing taxa from the Proteobacteria, Firmicutes, Actinobacteria and Bacteroidetes phyla. A total of 40 rhizosphere and phyllosphere bacteria were isolated from different cereal crops This research contributes to our understanding of microbial dynamics within cereal crop ecosystems and informs strategies for optimizing agricultural practices to meet the challenges of global food security and environmental sustainability.
Keywords: Rhizosphere, bacteria, phyllosphere bacteria, isolation, biofertilizers, environmental sustainability, microbial dynamics, cereal crop, bacteroidetes