Association of Transcription Factor 7 Like 2 (TCF7L2) rs12255372 (G/T) Gene Polymorphism and Type 2 Diabetes Mellitus
Mahmood Hassan Dalhat *
Department of Biochemistry, Usmanu Danfodiyo University Sokoto, Nigeria
Ibrahim Bashiru
Department of Biochemistry, Sokoto State University, Nigeria
Haris Ja’afar Bello
Department of Biosciences, COMSATS Institute of Information Technology, Islamabad, Pakistan
Yusuf Saidu
Department of Biochemistry, Usmanu Danfodiyo University Sokoto, Nigeria
Abdullahi Yahya Abbas
Department of Biochemistry, Usmanu Danfodiyo University Sokoto, Nigeria
*Author to whom correspondence should be addressed.
Abstract
Association of Transcription Factor 7 Like 2 (TCF7L2) rs12255372 (G/T) Gene Polymorphism and Type 2 Diabetes Mellitus
Aim: The rs12255372 of transcription factor 7 like 2 (TCF7L2) was found to be associated with risk of type 2 diabetes mellitus (T2DM) in different populations worldwide. Therefore in the present study we study the relationship between rs12255372 and T2DM in Pakistani population.
Methodology: The study comprised of 333 T2DM subjects and 234 normoglycemic control. Genotyping was done using polymerase chain reaction-restriction fragment length polymorphism (PCR-RFLP).
Results: Logistic regression analysis of allotyped data revealed association of rs12255372 with T2DM (odd ratio [OR] =2.30; 95% confidence interval [CI] 1.68-3.16, P=3.14× 10-8), also significant association (OR=3.03; 95% CI 2.07-4.43, P=1.00× 10-9) was observed in the dominant model (DM). Upon stratified analysis we observed T alleles in females (OR=3.60; 95% CI 1.94-7.13, P=6.70× 10-6) to be more associated with the disease compared to males (OR=1.94; 95% CI 1.32-2.87, P=4.56× 10-4). GT genotypes of both genders support their allelotypes.
Conclusion: The T allele and GT genotype highlight the possible role of rs12255372 in Pakistani T2DM patients.
Keywords: T2DM, TCF7L2, polymorphism, Wnt