Evaluation of Bacillus thuringiensis Snkr10 as a Potent Plant Growth Promoting Phyllospheric Bacterium Isolated from Spinach (Spinacia oleracea) Plants
Nisha Sharma
Microbial Resource Technology Laboratory, Department of Microbiology, Kurukshetra University, Kurukshetra, India
Baljeet Singh Saharan *
Microbial Resource Technology Laboratory, Department of Microbiology, Kurukshetra University, Kurukshetra, India
*Author to whom correspondence should be addressed.
Abstract
Evaluation of Bacillus thuringiensis Snkr10 as a Potent Plant Growth Promoting Phyllospheric Bacterium Isolated from Spinach (Spinacia oleracea) Plants
The phyllospheric microorganism used leaf surface of plants as habitat and help to maintain the plants growth by behaving as plant growth promoting bacteria (PGPB). SNKr10 is a gram positive and rod shaped spinach phyllospheric bacterium with 100% identity to B. thuringiensis (Accession no: KU569966). This bacterium showed various plant growth promoting (PGP) attributes like phosphate solubilization, Indole acetic acid (IAA), siderophore, NH3, HCN production and in vitro biological nitrogen fixation (BNF). Result also showed that this bacterial strain exhibit positive effect on the growth parameters of T. foenum-graecum (methi) under in vitro seed germination experiment and pot trial of V. radiate (mung bean). These results suggested that B. thuringensis has an excellent potential to be used as bio-inoculant for agriculture.
Keywords: IAA, in vitro seed germination assay, in vitro BNF, PGPB