Nutritional and Molecular Analysis of Wild Edible Gelam Mushroom (Boletus sp.) from Kelantan, Malaysia
Mohd Hafis Yuswan
Faculty of Science and Technology, School of Biosciences and Biotechnology, Universiti Kebangsaan Malaysia, Bangi, 43600, Selangor, Malaysia
Yue-Yuan On
Faculty of Science, Institute of Biological Sciences, University of Malaya, 50603 Kuala Lumpur, Malaysia
Ying-Ju Tan
Animal Science Research Centre, Malaysian Agricultural Research and Development Institute (MARDI), Persiaran MARDI-UPM, 43400 Serdang, Selangor, Malaysia
Wei-Hong Lai *
Clinical Research Centre, Level 3, Dermatology Block, Hospital Kuala Lumpur, 58600, Kuala Lumpur, Malaysia
Zainanda Zainal
Clinical Research Centre, Level 3, Dermatology Block, Hospital Kuala Lumpur, 58600, Kuala Lumpur, Malaysia
Jie-Xian Sz
Faculty of Science, Institute of Biological Sciences, University of Malaya, 50603 Kuala Lumpur, Malaysia
Fauzi Daud
Faculty of Science and Technology, School of Biosciences and Biotechnology, Universiti Kebangsaan Malaysia, Bangi, 43600, Selangor, Malaysia
*Author to whom correspondence should be addressed.
Abstract
Aims: To conduct nutritional and molecular analysis of Gelam mushroom which is believed to have contained medicinal properties.
Place and Duration of Study: School of Biosciences and Biotechnology, Faculty of Science and Technology, Universiti Kebangsaan Malaysia, between 2012 and 2014.
Methodology: The fruiting body of Gelam mushroom was collected from Bachok, Kelantan. Proximate composition and mineral content of the fruiting body of this mushroom was analyzed according to the guidelines of Association of Official Analytical Chemists and Alam et al. respectively. Fungal specific primers pairs ITS1-F and ITS4 were used in molecular analysis.
Results: Every 100 g of fruiting body containing 0.13 g fat, 4.53 g protein, 3.13 g fiber, 0.87 g ash, 5.1 g carbohydrate, 4.4 mg Ca, 297.13 mg K, 7.27 mg Mg and 24.4 mg Na. Molecular analysis using internal transcribed spacer (ITS) region of nuclear ribosomal deoxyribonucleic acid (DNA) on Gelam mushrooms collected from the state of Kelantan, Malaysia were identified to be from Boletus genus.
Conclusion: Present study demonstrated that Gelam mushroom could be a new Boletus species and is a potential source of food rich in minerals.
Keywords: Gelam mushroom, proximate analysis, minerals content, molecular analysis, Boletus sp